Hotspot

trnL-CAA-trnfM-CAU

IRb · 116950-118390 · 1441 bp

Back to Cyperus

Pi 0.0384

MarkerSeek score 67.60

Primer available yes

Label trnL-CAA-trnfM-CAU

Feature Metadata

label_name: trnL-CAA-trnfM-CAU
feature_type: igs
region: IRb
length_bp: 1441
variable_sites: 216
pi: 0.0384
unique_haplotype_count: 5
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 67.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnL-CAA-trnfM-CAU_p1	AAATGGTAGACACGCGAGAC	ATCCTCTCGTACCAGAGCTC	50.0	55.0	58.0	58.0	1666-1817 bp	0.875	77.0
trnL-CAA-trnfM-CAU_p2	TGAAATGGTAGACACGCGAG	ATCCTCTCGTACCAGAGCTC	50.0	55.0	58.0	58.0	1668-1819 bp	0.875	77.0
trnL-CAA-trnfM-CAU_p3	AAATGGTAGACACGCGAGAC	CAGAGCTCTCGTACCAGAGA	50.0	55.0	58.0	58.0	1654-1805 bp	0.875	77.0
trnL-CAA-trnfM-CAU_p4	AAATGGTAGACACGCGAGAC	CCTCAGCCTACGGGGTATTA	50.0	55.0	58.0	58.0	2246-2397 bp	0.875	76.6
trnL-CAA-trnfM-CAU_p5	TGAAATGGTAGACACGCGAG	CCTCAGCCTACGGGGTATTA	50.0	55.0	58.0	58.0	2248-2399 bp	0.875	76.6