Hotspot

ndhJ-trnF-GAA

LSC · 50251-50890 · 640 bp

Back to Cyperus

Pi 0.0185

MarkerSeek score 62.70

Primer available yes

Label ndhJ-trnF-GAA

Feature Metadata

label_name: ndhJ-trnF-GAA
feature_type: igs
region: LSC
length_bp: 640
variable_sites: 35
pi: 0.0185
unique_haplotype_count: 6
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 62.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhJ-trnF-GAA_p1	TGAAAGTTGGATAGGCTGGC	TCGTGTCACCAGTTCGAATC	50.0	50.0	57.9	57.9	677-777 bp	1.0	86.3
ndhJ-trnF-GAA_p2	TGAAAGTTGGATAGGCTGGC	CGTGTCACCAGTTCGAATCT	50.0	50.0	57.9	57.9	676-776 bp	1.0	86.3
ndhJ-trnF-GAA_p3	TGAAAGTTGGATAGGCTGGC	CTCGTGTCACCAGTTCGAAT	50.0	50.0	57.9	57.9	678-778 bp	1.0	86.3
ndhJ-trnF-GAA_p4	TGAAAGTTGGATAGGCTGGC	GTGGTAGAGCAGAGGACTGA	50.0	55.0	57.9	58.2	703-803 bp	1.0	85.4
ndhJ-trnF-GAA_p5	TGAAAGTTGGATAGGCTGGC	GGGATAGCTCAGGTGGTAGA	50.0	55.0	57.9	57.6	715-815 bp	1.0	83.6