Hotspot

trnH-GUG-psbA

LSC · 127-1012 · 886 bp

Back to Cyperus

Pi 0.019

MarkerSeek score 66.50

Primer available yes

Label trnH-GUG-psbA

Feature Metadata

label_name: trnH-GUG-psbA
feature_type: igs
region: LSC
length_bp: 886
variable_sites: 54
pi: 0.019
unique_haplotype_count: 7
primer_available: yes
alignment_reliability: 0.98
markerseek_score: 66.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnH-GUG-psbA_p1	GATCCACTTGGCTACATCCG	GCTGTTGTTGAAGTTCCTGC	55.0	50.0	58.1	57.9	912-942 bp	1.0	86.5
trnH-GUG-psbA_p2	CAATCCACTGCCTTGATCCA	GCTGTTGTTGAAGTTCCTGC	50.0	50.0	57.9	57.9	926-956 bp	1.0	86.3
trnH-GUG-psbA_p3	AATCCACTGCCTTGATCCAC	GCTGTTGTTGAAGTTCCTGC	50.0	50.0	57.9	57.9	925-955 bp	1.0	86.3
trnH-GUG-psbA_p4	ACAATCCACTGCCTTGATCC	GCTGTTGTTGAAGTTCCTGC	50.0	50.0	57.9	57.9	927-957 bp	1.0	86.3
trnH-GUG-psbA_p5	GATCCACAATCCACTGCCTT	GCTGTTGTTGAAGTTCCTGC	50.0	50.0	57.9	57.9	932-962 bp	1.0	86.2