primer_id	label_name	rank	fwd_seq	rev_seq	fwd_len	rev_len	fwd_gc	rev_gc	fwd_tm	rev_tm	fwd_self_any_th	rev_self_any_th	primer3_penalty	target_start	target_end	amplicon_size	cross_species_success_rate	primer_score
trnH-GUG-psbA_p1	trnH-GUG-psbA	1	GATCCACTTGGCTACATCCG	GCTGTTGTTGAAGTTCCTGC	20	20	55.0	50.0	58.1	57.9	0.0	0.0	0.254381	127	1012	912-942	1.000000	86.5
trnH-GUG-psbA_p2	trnH-GUG-psbA	2	CAATCCACTGCCTTGATCCA	GCTGTTGTTGAAGTTCCTGC	20	20	50.0	50.0	57.9	57.9	0.0	0.0	0.258743	127	1012	926-956	1.000000	86.3
trnH-GUG-psbA_p3	trnH-GUG-psbA	3	AATCCACTGCCTTGATCCAC	GCTGTTGTTGAAGTTCCTGC	20	20	50.0	50.0	57.9	57.9	0.0	0.0	0.260250	127	1012	925-955	1.000000	86.3
trnH-GUG-psbA_p4	trnH-GUG-psbA	4	ACAATCCACTGCCTTGATCC	GCTGTTGTTGAAGTTCCTGC	20	20	50.0	50.0	57.9	57.9	0.0	0.0	0.260250	127	1012	927-957	1.000000	86.3
trnH-GUG-psbA_p5	trnH-GUG-psbA	5	GATCCACAATCCACTGCCTT	GCTGTTGTTGAAGTTCCTGC	20	20	50.0	50.0	57.9	57.9	0.0	0.0	0.260250	127	1012	932-962	1.000000	86.2
trnY-GUA-trnD-GUC_p1	trnY-GUA-trnD-GUC	1	TTAATCCTTCCACCCAGGGT	TCGGATTCTAGTTCGGGACT	20	20	50.0	50.0	57.9	57.6	4.8	0.0	0.500785	32627	33184	684-745	1.000000	84.1
trnY-GUA-trnD-GUC_p2	trnY-GUA-trnD-GUC	2	TTAATCCTTCCACCCAGGGT	CGGATTCTAGTTCGGGACTG	20	20	50.0	55.0	57.9	57.5	4.8	0.0	0.562544	32627	33184	683-744	1.000000	83.6
trnY-GUA-trnD-GUC_p3	trnY-GUA-trnD-GUC	3	TTAATCCTTCCACCCAGGGT	GCGGTGCTCTAACCAATTGA	20	20	50.0	50.0	57.9	58.6	4.8	7.1	0.619847	32627	33184	628-689	1.000000	83.4
trnY-GUA-trnD-GUC_p4	trnY-GUA-trnD-GUC	4	GGGGACGGACTGTAAATTCG	TCGGATTCTAGTTCGGGACT	20	20	55.0	50.0	58.4	57.6	0.0	0.0	0.783450	32627	33184	645-706	1.000000	82.0
trnY-GUA-trnD-GUC_p5	trnY-GUA-trnD-GUC	5	GGGACGGACTGTAAATTCGT	TCGGATTCTAGTTCGGGACT	20	20	50.0	50.0	57.6	57.6	0.0	0.0	0.807127	32627	33184	644-705	1.000000	81.8
ndhJ-trnF-GAA_p1	ndhJ-trnF-GAA	1	TGAAAGTTGGATAGGCTGGC	TCGTGTCACCAGTTCGAATC	20	20	50.0	50.0	57.9	57.9	0.0	0.0	0.189096	50251	50890	677-777	1.000000	86.3
ndhJ-trnF-GAA_p2	ndhJ-trnF-GAA	2	TGAAAGTTGGATAGGCTGGC	CGTGTCACCAGTTCGAATCT	20	20	50.0	50.0	57.9	57.9	0.0	0.0	0.194763	50251	50890	676-776	1.000000	86.3
ndhJ-trnF-GAA_p3	ndhJ-trnF-GAA	3	TGAAAGTTGGATAGGCTGGC	CTCGTGTCACCAGTTCGAAT	20	20	50.0	50.0	57.9	57.9	0.0	0.0	0.194763	50251	50890	678-778	1.000000	86.3
ndhJ-trnF-GAA_p4	ndhJ-trnF-GAA	4	TGAAAGTTGGATAGGCTGGC	GTGGTAGAGCAGAGGACTGA	20	20	50.0	55.0	57.9	58.2	0.0	0.0	0.288034	50251	50890	703-803	1.000000	85.4
ndhJ-trnF-GAA_p5	ndhJ-trnF-GAA	5	TGAAAGTTGGATAGGCTGGC	GGGATAGCTCAGGTGGTAGA	20	20	50.0	55.0	57.9	57.6	0.0	0.0	0.512213	50251	50890	715-815	1.000000	83.6
rps12-psbB_p1	rps12-psbB	1	GGACATCCTTTAAGAGCGGG	CAATCGACCGGGATCATTCA	20	20	55.0	50.0	58.0	57.8	0.0	6.3	0.284015	73424	75224	1915-2052	1.000000	82.2
rps12-psbB_p2	rps12-psbB	2	GGACATCCTTTAAGAGCGGG	TAAAGCCATTGAACCAGCCC	20	20	55.0	50.0	58.0	58.4	0.0	0.0	0.489106	73424	75224	1975-2112	1.000000	80.5
rps12-psbB_p3	rps12-psbB	3	GGACATCCTTTAAGAGCGGG	GAACAGAAAGCAATCGACCG	20	20	55.0	50.0	58.0	57.5	0.0	0.0	0.555688	73424	75224	1925-2062	1.000000	80.0
rps12-psbB_p4	rps12-psbB	4	GGACATCCTTTAAGAGCGGG	GCAACTAGAGCTGTGTGCAT	20	20	55.0	50.0	58.0	58.6	0.0	10.0	0.598166	73424	75224	1950-2087	1.000000	79.6
rps12-psbB_p5	rps12-psbB	5	GGACATCCTTTAAGAGCGGG	AACAGAAAGCAATCGACCGG	20	20	55.0	50.0	58.0	58.8	0.0	0.0	0.888337	73424	75224	1924-2061	1.000000	77.4
ndhB-trnL-CAA_p1	ndhB-trnL-CAA	1	TGCAATTGCTCAGGATACCC	GTCTCGCGTGTCTACCATTT	20	20	50.0	50.0	57.9	58.0	29.4	5.8	0.064968	116093	116868	836-2507	0.875000	74.7
ndhB-trnL-CAA_p2	ndhB-trnL-CAA	2	TGCAATTGCTCAGGATACCC	CTCGCGTGTCTACCATTTCA	20	20	50.0	50.0	57.9	58.0	29.4	5.8	0.066338	116093	116868	834-2505	0.875000	74.7
ndhB-trnL-CAA_p3	ndhB-trnL-CAA	3	TGCAATTGCTCAGGATACCC	TGTTTGAACAGCGTCAGTCA	20	20	50.0	45.0	57.9	58.0	29.4	0.0	0.074275	116093	116868	1374-2551	0.625000	65.0
ndhB-trnL-CAA_p4	ndhB-trnL-CAA	4	TGCAATTGCTCAGGATACCC	TCCATAACCGCAAGTGCTAC	20	20	50.0	50.0	57.9	58.0	29.4	1.2	0.075679	116093	116868	1421-2598	0.625000	64.9
ndhB-trnL-CAA_p5	ndhB-trnL-CAA	5	TGCAATTGCTCAGGATACCC	ATAACCGCAAGTGCTACAGG	20	20	50.0	50.0	57.9	58.0	29.4	1.2	0.081313	116093	116868	1418-2595	0.625000	64.9
trnL-CAA_p1	trnL-CAA	1	TGTTCCGCCCAAAATGAGAA	GTGTGGGGAGGATGATTTCC	20	20	45.0	55.0	58.0	57.9	0.0	0.0	0.070299	116869	116949	1018-2689	0.875000	74.8
trnL-CAA_p2	trnL-CAA	2	TGTTCCGCCCAAAATGAGAA	TGTTTGAACAGCGTCAGTCA	20	20	45.0	45.0	58.0	58.0	0.0	0.0	0.028845	116869	116949	1282-2459	0.625000	65.3
trnL-CAA_p3	trnL-CAA	3	TGTTCCGCCCAAAATGAGAA	TCCATAACCGCAAGTGCTAC	20	20	45.0	50.0	58.0	58.0	0.0	1.2	0.030248	116869	116949	1329-2506	0.625000	65.2
trnL-CAA_p4	trnL-CAA	4	TGTTCCGCCCAAAATGAGAA	ATAACCGCAAGTGCTACAGG	20	20	45.0	50.0	58.0	58.0	0.0	1.2	0.035882	116869	116949	1326-2503	0.625000	65.2
trnL-CAA_p5	trnL-CAA	5	TGTTCCGCCCAAAATGAGAA	AGCGTCAGTCAAACCTGTTT	20	20	45.0	45.0	58.0	58.0	0.0	0.0	0.050093	116869	116949	1273-2450	0.625000	65.1
trnL-CAA-trnfM-CAU_p1	trnL-CAA-trnfM-CAU	1	AAATGGTAGACACGCGAGAC	ATCCTCTCGTACCAGAGCTC	20	20	50.0	55.0	58.0	58.0	15.9	11.7	0.032171	116950	118390	1666-1817	0.875000	77.0
trnL-CAA-trnfM-CAU_p2	trnL-CAA-trnfM-CAU	2	TGAAATGGTAGACACGCGAG	ATCCTCTCGTACCAGAGCTC	20	20	50.0	55.0	58.0	58.0	15.9	11.7	0.033541	116950	118390	1668-1819	0.875000	77.0
trnL-CAA-trnfM-CAU_p3	trnL-CAA-trnfM-CAU	3	AAATGGTAGACACGCGAGAC	CAGAGCTCTCGTACCAGAGA	20	20	50.0	55.0	58.0	58.0	15.9	20.2	0.039089	116950	118390	1654-1805	0.875000	77.0
trnL-CAA-trnfM-CAU_p4	trnL-CAA-trnfM-CAU	4	AAATGGTAGACACGCGAGAC	CCTCAGCCTACGGGGTATTA	20	20	50.0	55.0	58.0	58.0	15.9	0.0	0.013201	116950	118390	2246-2397	0.875000	76.6
trnL-CAA-trnfM-CAU_p5	trnL-CAA-trnfM-CAU	5	TGAAATGGTAGACACGCGAG	CCTCAGCCTACGGGGTATTA	20	20	50.0	55.0	58.0	58.0	15.9	0.0	0.014571	116950	118390	2248-2399	0.875000	76.6
trnR-ACG-trnN-GUU_p1	trnR-ACG-trnN-GUU	1	TTCCTTTTTGAGGGTGCTCC	CCGTTGTTCCTCAGTAGCTC	20	20	50.0	55.0	58.0	58.0	0.0	0.0	0.006955	126674	128892	2406-2538	0.750000	71.3
trnR-ACG-trnN-GUU_p2	trnR-ACG-trnN-GUU	2	TAACTCGACGCCAGAATGTG	CCGTTGTTCCTCAGTAGCTC	20	20	50.0	55.0	58.0	58.0	0.0	0.0	0.011532	126674	128892	2703-2835	0.750000	71.1
trnR-ACG-trnN-GUU_p3	trnR-ACG-trnN-GUU	3	TTCCTTTTTGAGGGTGCTCC	AATGAGACATGGAAGGGGGA	20	20	50.0	50.0	58.0	58.0	0.0	0.0	0.019002	126674	128892	2521-2653	0.750000	71.1
trnR-ACG-trnN-GUU_p4	trnR-ACG-trnN-GUU	4	CGAAATCACATGGCTGAGGA	CCGTTGTTCCTCAGTAGCTC	20	20	50.0	55.0	58.0	58.0	0.0	0.0	0.030751	126674	128892	2430-2562	0.750000	71.1
trnR-ACG-trnN-GUU_p5	trnR-ACG-trnN-GUU	5	TAACTCGACGCCAGAATGTG	AATGAGACATGGAAGGGGGA	20	20	50.0	50.0	58.0	58.0	0.0	0.0	0.023579	126674	128892	2818-2950	0.750000	71.0
trnN-GUU-rps15_p1	trnN-GUU-rps15	1	CGGGTTCAGGGAGGTAAATG	ACATTTCGTCCCCAAGAACC	20	20	55.0	50.0	58.0	58.1	0.0	0.0	0.132749	130237	130356	1076-1093	1.000000	90.9
trnN-GUU-rps15_p2	trnN-GUU-rps15	2	GATGGGGAACTCGCTTTAGG	ACATTTCGTCCCCAAGAACC	20	20	55.0	50.0	58.0	58.1	0.0	0.0	0.139436	130237	130356	1013-1030	1.000000	90.9
trnN-GUU-rps15_p3	trnN-GUU-rps15	3	GTTCAGGGAGGTAAATGCGT	ACATTTCGTCCCCAAGAACC	20	20	50.0	50.0	57.9	58.1	0.0	0.0	0.203787	130237	130356	1073-1090	1.000000	90.4
trnN-GUU-rps15_p4	trnN-GUU-rps15	4	GCTTATATGCCATGGGAGGG	ACATTTCGTCCCCAAGAACC	20	20	55.0	50.0	57.9	58.1	15.1	0.0	0.229152	130237	130356	977-994	1.000000	90.2
trnN-GUU-rps15_p5	trnN-GUU-rps15	5	CGGGTTCAGGGAGGTAAATG	CATTTCGTCCCCAAGAACCT	20	20	55.0	50.0	58.0	57.8	0.0	0.0	0.238760	130237	130356	1075-1092	1.000000	90.1
trnT-UGU-ccsA_p1	trnT-UGU-ccsA	1	TCGGATTCTGGATCACGAGA	CAGCTCTTTTATGCGGGTCT	20	20	50.0	50.0	57.9	58.0	0.0	0.0	0.082621	140401	141692	2126-2597	0.875000	81.5
trnT-UGU-ccsA_p2	trnT-UGU-ccsA	2	CTCAGAGGTTAGAGCATCGC	CAGCTCTTTTATGCGGGTCT	20	20	55.0	50.0	57.9	58.0	0.0	0.0	0.162578	140401	141692	1674-2132	0.875000	81.4
trnT-UGU-ccsA_p3	trnT-UGU-ccsA	3	TCGGATTCTGGATCACGAGA	GCGACATCAGGTTTCTGTCT	20	20	50.0	50.0	57.9	57.9	0.0	0.0	0.138837	140401	141692	2238-2709	0.875000	81.0
trnT-UGU-ccsA_p4	trnT-UGU-ccsA	4	TTGCATTACCAGGACTGAGC	CAGCTCTTTTATGCGGGTCT	20	20	50.0	50.0	57.9	58.0	0.0	0.0	0.143699	140401	141692	2338-2809	0.875000	80.9
trnT-UGU-ccsA_p5	trnT-UGU-ccsA	5	TCGGATTCTGGATCACGAGA	GTACCTGCACTGCAATCTCA	20	20	50.0	50.0	57.9	57.9	0.0	5.0	0.151923	140401	141692	2187-2658	0.875000	80.9
trnL-UAG-rpl32_p1	trnL-UAG-rpl32	1	ACACTAGGCACTGCTTCCTA	TACTGGGTCCGGGGTTTTAT	20	20	50.0	50.0	58.1	58.0	0.0	0.0	0.091703	142807	143587	1318-1970	0.875000	82.8
trnL-UAG-rpl32_p2	trnL-UAG-rpl32	2	TACACTAGGCACTGCTTCCT	TACTGGGTCCGGGGTTTTAT	20	20	50.0	50.0	58.1	58.0	0.0	0.0	0.091703	142807	143587	1319-1971	0.875000	82.7
trnL-UAG-rpl32_p3	trnL-UAG-rpl32	3	CCTGATGTCGCAAAAGCTTG	TACTGGGTCCGGGGTTTTAT	20	20	50.0	50.0	58.0	58.0	0.0	0.0	0.057149	142807	143587	1759-2411	0.875000	82.4
trnL-UAG-rpl32_p4	trnL-UAG-rpl32	4	AGACCCGCATAAAAGAGCTG	TACTGGGTCCGGGGTTTTAT	20	20	50.0	50.0	58.0	58.0	0.0	0.0	0.060126	142807	143587	1880-2532	0.875000	82.3
trnL-UAG-rpl32_p5	trnL-UAG-rpl32	5	CCTGATGTCGCAAAAGCTTG	TCGAATCCTTCTACCTCCCC	20	20	50.0	55.0	58.0	57.9	0.0	0.0	0.110490	142807	143587	1890-2542	0.875000	82.0
rpl32-ndhF_p1	rpl32-ndhF	1	GCTAAAGAAGAGGCTTTTACTGC	CACTTTGATGGAAGCGGACT	23	20	43.5	50.0	58.0	58.2	0.0	0.0	3.210698	143780	145083	1930-2577	0.875000	54.6
rpl32-ndhF_p2	rpl32-ndhF	2	AAGAAGAGGCTTTTACTGCA	CACTTTGATGGAAGCGGACT	20	20	40.0	50.0	54.9	58.2	0.0	0.0	3.311137	143780	145083	1926-2573	0.875000	53.8
rpl32-ndhF_p3	rpl32-ndhF	3	AGAAGAGGCTTTTACTGCAA	CACTTTGATGGAAGCGGACT	20	20	40.0	50.0	54.9	58.2	0.0	0.0	3.311137	143780	145083	1925-2572	0.875000	53.8
rpl32-ndhF_p4	rpl32-ndhF	4	GCTAAAGAAGAGGCTTTTACTGC	TCACTTTGATGGAAGCGGAC	23	20	43.5	50.0	58.0	58.2	0.0	0.0	3.215801	143780	145083	1931-1940	0.750000	48.0
rpl32-ndhF_p5	rpl32-ndhF	5	AGAAGAGGCTTTTACTGCAA	TCACTTTGATGGAAGCGGAC	20	20	40.0	50.0	54.9	58.2	0.0	0.0	3.316240	143780	145083	1926-1935	0.750000	47.2
rps15-trnN-GUU_p1	rps15-trnN-GUU	1	ACATTTCGTCCCCAAGAACC	CGGGTTCAGGGAGGTAAATG	20	20	50.0	55.0	58.1	58.0	0.0	0.0	0.132749	154552	154671	1076-1093	1.000000	90.9
rps15-trnN-GUU_p2	rps15-trnN-GUU	2	ACATTTCGTCCCCAAGAACC	GATGGGGAACTCGCTTTAGG	20	20	50.0	55.0	58.1	58.0	0.0	0.0	0.139436	154552	154671	1013-1030	1.000000	90.9
rps15-trnN-GUU_p3	rps15-trnN-GUU	3	ACATTTCGTCCCCAAGAACC	GTTCAGGGAGGTAAATGCGT	20	20	50.0	50.0	58.1	57.9	0.0	0.0	0.203787	154552	154671	1073-1090	1.000000	90.4
rps15-trnN-GUU_p4	rps15-trnN-GUU	4	ACATTTCGTCCCCAAGAACC	GCTTATATGCCATGGGAGGG	20	20	50.0	55.0	58.1	57.9	0.0	15.1	0.229152	154552	154671	977-994	1.000000	90.2
rps15-trnN-GUU_p5	rps15-trnN-GUU	5	CATTTCGTCCCCAAGAACCT	CGGGTTCAGGGAGGTAAATG	20	20	50.0	55.0	57.8	58.0	0.0	0.0	0.238760	154552	154671	1075-1092	1.000000	90.1
trnN-GUU-trnR-ACG_p1	trnN-GUU-trnR-ACG	1	CCGTTGTTCCTCAGTAGCTC	TTCCTTTTTGAGGGTGCTCC	20	20	55.0	50.0	58.0	58.0	0.0	0.0	0.006955	156016	158234	2406-2538	0.750000	74.4
trnN-GUU-trnR-ACG_p2	trnN-GUU-trnR-ACG	2	CCGTTGTTCCTCAGTAGCTC	TAACTCGACGCCAGAATGTG	20	20	55.0	50.0	58.0	58.0	0.0	0.0	0.011532	156016	158234	2703-2835	0.750000	74.3
trnN-GUU-trnR-ACG_p3	trnN-GUU-trnR-ACG	3	AATGAGACATGGAAGGGGGA	TTCCTTTTTGAGGGTGCTCC	20	20	50.0	50.0	58.0	58.0	0.0	0.0	0.019002	156016	158234	2521-2653	0.750000	74.3
trnN-GUU-trnR-ACG_p4	trnN-GUU-trnR-ACG	4	AATGAGACATGGAAGGGGGA	TAACTCGACGCCAGAATGTG	20	20	50.0	50.0	58.0	58.0	0.0	0.0	0.023579	156016	158234	2818-2950	0.750000	74.2
trnN-GUU-trnR-ACG_p5	trnN-GUU-trnR-ACG	5	CCGTTGTTCCTCAGTAGCTC	CGAAATCACATGGCTGAGGA	20	20	55.0	50.0	58.0	58.0	0.0	0.0	0.030751	156016	158234	2430-2562	0.750000	74.2