Hotspot

rpoC1

LSC · 20870-23735 · 2866 bp

Back to Cynometra

Pi 0.00182

MarkerSeek score 58.30

Primer available yes

Label rpoC1

Feature Metadata

label_name: rpoC1
feature_type: gene
region: LSC
length_bp: 2866
variable_sites: 10
pi: 0.00182
unique_haplotype_count: 4
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 58.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpoC1_p1	GAGAGACCCGAATTCACACC	TCCAGATTCATAGGAAGGAAGC	55.0	45.5	58.0	57.3	2943-2979 bp	1.0	59.6
rpoC1_p2	GGAGAGACCCGAATTCACAC	TCCAGATTCATAGGAAGGAAGC	55.0	45.5	58.0	57.3	2944-2980 bp	1.0	59.6
rpoC1_p3	GAGAGACCCGAATTCACACC	CCAGATTCATAGGAAGGAAGCT	55.0	45.5	58.0	57.3	2942-2978 bp	1.0	59.5
rpoC1_p4	GGAGAGACCCGAATTCACAC	CCAGATTCATAGGAAGGAAGCT	55.0	45.5	58.0	57.3	2943-2979 bp	1.0	59.5
rpoC1_p5	GATCCTAGTTGACCCGGAGA	TCCAGATTCATAGGAAGGAAGC	55.0	45.5	57.9	57.3	2959-2995 bp	1.0	59.1