Hotspot

rps15-ycf1

SSC · 127202-127632 · 431 bp

Back to Cynometra

Pi 0.0171

MarkerSeek score 45.50

Primer available yes

Label rps15-ycf1

Feature Metadata

label_name: rps15-ycf1
feature_type: igs
region: SSC
length_bp: 431
variable_sites: 12
pi: 0.0171
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 0.98
markerseek_score: 45.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rps15-ycf1_p1	GGTGCTGAGGACCCATATTG	GCAGCCGTTTCAGTATGGTA	55.0	50.0	58.0	58.0	912-920 bp	1.0	82.0
rps15-ycf1_p2	CTTGTAGCTGGGGCATTCAT	GCAGCCGTTTCAGTATGGTA	50.0	50.0	57.9	58.0	874-882 bp	1.0	81.8
rps15-ycf1_p3	TTACGGTTATTGCTTCGGGG	GCAGCCGTTTCAGTATGGTA	50.0	50.0	58.0	58.0	1104-1112 bp	1.0	81.6
rps15-ycf1_p4	ATGAGTCGAAGAACACCGTG	GCAGCCGTTTCAGTATGGTA	50.0	50.0	57.9	58.0	940-948 bp	1.0	81.6
rps15-ycf1_p5	TCCTAACTGTTCCGGTCCAT	GCAGCCGTTTCAGTATGGTA	50.0	50.0	58.1	58.0	1124-1132 bp	1.0	81.3