Hotspot

rpl20-rps12

LSC · 70879-71647 · 769 bp

Back to Cynometra

Pi 0.00305
MarkerSeek score 60.50
Primer available yes
Label rpl20-rps12

Feature Metadata

label_name
rpl20-rps12
feature_type
igs
region
LSC
length_bp
769
variable_sites
4
pi
0.00305
unique_haplotype_count
4
primer_available
yes
alignment_reliability
1.00
markerseek_score
60.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
rpl20-rps12_p1 GTCCGATGTTTCCGAGCTAT CGCCGAGGAACATGTACTAG 50.0 55.0 57.5 57.9 837-840 bp 1.0 75.6
rpl20-rps12_p2 TGTCCGATGTTTCCGAGCTA CGCCGAGGAACATGTACTAG 50.0 55.0 58.8 57.9 838-841 bp 1.0 73.1
rpl20-rps12_p3 TTTGTCCGATGTTTCCGAGC CGCCGAGGAACATGTACTAG 50.0 55.0 58.8 57.9 840-843 bp 1.0 72.9
rpl20-rps12_p4 GTCCGATGTTTCCGAGCTAT GCCGAGGAACATGTACTAGG 50.0 55.0 57.5 57.5 836-839 bp 1.0 72.5
rpl20-rps12_p5 AGTCTTGACCGAAAGCTTGA CGCCGAGGAACATGTACTAG 45.0 55.0 57.1 57.9 876-879 bp 1.0 72.4