Hotspot

petA-psbJ

LSC · 64964-65791 · 828 bp

Back to Cynometra

Pi 0.0111

MarkerSeek score 64.60

Primer available yes

Label petA-psbJ

Feature Metadata

label_name: petA-psbJ
feature_type: igs
region: LSC
length_bp: 828
variable_sites: 17
pi: 0.0111
unique_haplotype_count: 4
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 64.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
petA-psbJ_p1	CGTGTCCAAGGTCTCTTGTT	ATTCCGGATTGGGTTCATCC	50.0	50.0	57.8	57.6	963-965 bp	1.0	76.9
petA-psbJ_p2	CGTTACGTGTCCAAGGTCTC	ATTCCGGATTGGGTTCATCC	55.0	50.0	58.3	57.6	968-970 bp	1.0	76.4
petA-psbJ_p3	GATCCGTTACGTGTCCAAGG	ATTCCGGATTGGGTTCATCC	55.0	50.0	58.4	57.6	972-974 bp	1.0	75.9
petA-psbJ_p4	ACGTGTCCAAGGTCTCTTGT	ATTCCGGATTGGGTTCATCC	50.0	50.0	58.9	57.6	964-966 bp	1.0	71.8
petA-psbJ_p5	ATCCGTTACGTGTCCAAGGT	ATTCCGGATTGGGTTCATCC	50.0	50.0	59.0	57.6	971-973 bp	1.0	70.6