Hotspot

accD-psaI

LSC · 61201-61777 · 577 bp

Back to Cynometra

Pi 0.0148

MarkerSeek score 65.30

Primer available yes

Label accD-psaI

Feature Metadata

label_name: accD-psaI
feature_type: igs
region: LSC
length_bp: 577
variable_sites: 15
pi: 0.0148
unique_haplotype_count: 4
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 65.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
accD-psaI_p1	CAATCGTACCGCGTAAACCT	GCCATTGCAATTGACGGAAA	50.0	45.0	58.4	57.9	743-812 bp	1.0	78.2
accD-psaI_p2	AATCGTACCGCGTAAACCTT	GCCATTGCAATTGACGGAAA	45.0	45.0	57.6	57.9	742-811 bp	1.0	78.2
accD-psaI_p3	ATCGTACCGCGTAAACCTTT	GCCATTGCAATTGACGGAAA	45.0	45.0	57.6	57.9	741-810 bp	1.0	78.2
accD-psaI_p4	AGGGCGTTCTGAGTGAGTTA	GCCATTGCAATTGACGGAAA	50.0	45.0	58.4	57.9	719-788 bp	1.0	78.2
accD-psaI_p5	TCAATCGTACCGCGTAAACC	GCCATTGCAATTGACGGAAA	50.0	45.0	58.4	57.9	744-813 bp	1.0	78.2