Hotspot

trnT-UGU-trnL-UAA

LSC · 48613-49358 · 746 bp

Back to Cynometra

Pi 0.00737

MarkerSeek score 66.40

Primer available yes

Label trnT-UGU-trnL-UAA

Feature Metadata

label_name: trnT-UGU-trnL-UAA
feature_type: igs
region: LSC
length_bp: 746
variable_sites: 10
pi: 0.00737
unique_haplotype_count: 4
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 66.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnT-UGU-trnL-UAA_p1	GCGATGCTCTAACCTCTGAG	TAGCGTCTACCAATTTCGCC	55.0	50.0	57.9	58.1	799-843 bp	1.0	82.3
trnT-UGU-trnL-UAA_p2	TAGGGGAAAAGAAAAGCCGG	TAGCGTCTACCAATTTCGCC	50.0	50.0	57.8	58.1	856-900 bp	1.0	81.4
trnT-UGU-trnL-UAA_p3	GGAATCGAACCGATGACCAT	TAGCGTCTACCAATTTCGCC	50.0	50.0	57.8	58.1	831-875 bp	1.0	81.3
trnT-UGU-trnL-UAA_p4	GCGATGCTCTAACCTCTGAG	AGTCCGTAGCGTCTACCAAT	55.0	50.0	57.9	58.2	805-849 bp	1.0	80.9
trnT-UGU-trnL-UAA_p5	TAGGGGAAAAGAAAAGCCGG	AGTCCGTAGCGTCTACCAAT	50.0	50.0	57.8	58.2	862-906 bp	1.0	80.0