Hotspot

psbZ-trnG-GCC

LSC · 37987-38673 · 687 bp

Back to Cynometra

Pi 0.0152

MarkerSeek score 64.40

Primer available yes

Label psbZ-trnG-GCC

Feature Metadata

label_name: psbZ-trnG-GCC
feature_type: igs
region: LSC
length_bp: 687
variable_sites: 18
pi: 0.0152
unique_haplotype_count: 4
primer_available: yes
alignment_reliability: 0.98
markerseek_score: 64.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbZ-trnG-GCC_p1	CTTCTCCTGATGGTTGGTCG	CGTCTTCTCCTTGGCAAAGA	55.0	50.0	58.0	57.8	841-881 bp	1.0	82.1
psbZ-trnG-GCC_p2	TTGCTTCTCCTGATGGTTGG	CGTCTTCTCCTTGGCAAAGA	50.0	50.0	57.8	57.8	844-884 bp	1.0	80.6
psbZ-trnG-GCC_p3	TCCTGATGGTTGGTCGAGTA	CGTCTTCTCCTTGGCAAAGA	50.0	50.0	57.8	57.8	837-877 bp	1.0	80.5
psbZ-trnG-GCC_p4	CTTCTCCTGATGGTTGGTCG	GGATAGCGGGAATTGAACCC	55.0	55.0	58.0	58.4	862-902 bp	1.0	80.2
psbZ-trnG-GCC_p5	GCTTCTCCTGATGGTTGGTC	CGTCTTCTCCTTGGCAAAGA	55.0	50.0	58.3	57.8	842-882 bp	1.0	80.2