Hotspot

trnE-UUC-trnT-GGU

LSC · 32315-33224 · 910 bp

Back to Cynometra

Pi 0.00526

MarkerSeek score 57.10

Primer available yes

Label trnE-UUC-trnT-GGU

Feature Metadata

label_name: trnE-UUC-trnT-GGU
feature_type: igs
region: LSC
length_bp: 910
variable_sites: 9
pi: 0.00526
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 0.97
markerseek_score: 57.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnE-UUC-trnT-GGU_p1	GCTGCCTCCTTGAAAGAGAG	GAACCGATGACTTACGCCTT	55.0	50.0	58.3	58.0	993-1026 bp	1.0	81.9
trnE-UUC-trnT-GGU_p2	GCTGCCTCCTTGAAAGAGAG	CCATGGCGTTACTCTACCAC	55.0	55.0	58.3	58.1	972-1005 bp	1.0	81.5
trnE-UUC-trnT-GGU_p3	GCTGCCTCCTTGAAAGAGAG	TGGCGTTACTCTACCACTGA	55.0	50.0	58.3	58.1	969-1002 bp	1.0	81.3
trnE-UUC-trnT-GGU_p4	GCTGCCTCCTTGAAAGAGAG	CCGATGACTTACGCCTTACC	55.0	55.0	58.3	58.2	990-1023 bp	1.0	80.7
trnE-UUC-trnT-GGU_p5	GCTGCCTCCTTGAAAGAGAG	TTGAACCGATGACTTACGCC	55.0	50.0	58.3	58.3	995-1028 bp	1.0	79.6