Hotspot

atpH-atpI

LSC · 13393-14595 · 1203 bp

Back to Cynometra

Pi 0.0036

MarkerSeek score 61.20

Primer available yes

Label atpH-atpI

Feature Metadata

label_name: atpH-atpI
feature_type: igs
region: LSC
length_bp: 1203
variable_sites: 8
pi: 0.0036
unique_haplotype_count: 4
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 61.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
atpH-atpI_p1	AATAGAAGCAAGCCCGACAG	TTTTGCAACTTTAGCTGCGG	50.0	45.0	58.0	57.9	1324-1325 bp	1.0	79.2
atpH-atpI_p2	ATAACGGAAGCGGCAGAAAT	TTTTGCAACTTTAGCTGCGG	45.0	45.0	58.0	57.9	1290-1291 bp	1.0	79.1
atpH-atpI_p3	AATAGAAGCAAGCCCGACAG	TCCCTGTCATGTTCCTTGGA	50.0	50.0	58.0	58.3	1373-1374 bp	1.0	78.2
atpH-atpI_p4	ATAACGGAAGCGGCAGAAAT	TCCCTGTCATGTTCCTTGGA	45.0	50.0	58.0	58.3	1339-1340 bp	1.0	78.1
atpH-atpI_p5	AATAACGGAAGCGGCAGAAA	TTTTGCAACTTTAGCTGCGG	45.0	45.0	58.2	57.9	1291-1292 bp	1.0	78.0