Hotspot

atpF-atpH

LSC · 12536-13146 · 611 bp

Back to Cynometra

Pi 0.0147

MarkerSeek score 63.10

Primer available yes

Label atpF-atpH

Feature Metadata

label_name: atpF-atpH
feature_type: igs
region: LSC
length_bp: 611
variable_sites: 15
pi: 0.0147
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 0.96
markerseek_score: 63.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
atpF-atpH_p1	TAAACCCGAAACTCTCAGCG	AGGCAGAGGGAAAAATACGG	50.0	50.0	57.9	57.6	769-794 bp	1.0	80.2
atpF-atpH_p2	CGTATGGCCAGTAACCCAAA	AGGCAGAGGGAAAAATACGG	50.0	50.0	57.9	57.6	751-776 bp	1.0	80.0
atpF-atpH_p3	TAAACCCGAAACTCTCAGCG	CCTGAGGCAGAGGGAAAAAT	50.0	50.0	57.9	57.5	773-798 bp	1.0	79.4
atpF-atpH_p4	CGTATGGCCAGTAACCCAAA	CCTGAGGCAGAGGGAAAAAT	50.0	50.0	57.9	57.5	755-780 bp	1.0	79.2
atpF-atpH_p5	TAAACCCGAAACTCTCAGCG	GCAGAGGGAAAAATACGGGG	50.0	55.0	57.9	58.6	767-792 bp	1.0	78.7