Hotspot

trnH-GUG-psbA

LSC · 87-339 · 253 bp

Back to Cynometra

Pi 0.027

MarkerSeek score 64.00

Primer available yes

Label trnH-GUG-psbA

Feature Metadata

label_name: trnH-GUG-psbA
feature_type: igs
region: LSC
length_bp: 253
variable_sites: 13
pi: 0.027
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 0.98
markerseek_score: 64.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnH-GUG-psbA_p1	GATCCACTTGGCTACATCCG	CCTCTAGACCTAGCTGCCAT	55.0	55.0	58.1	58.0	319-437 bp	1.0	82.7
trnH-GUG-psbA_p2	CAATCCACTGCCTTGATCCA	CCTCTAGACCTAGCTGCCAT	50.0	55.0	57.9	58.0	333-451 bp	1.0	82.5
trnH-GUG-psbA_p3	AATCCACTGCCTTGATCCAC	CCTCTAGACCTAGCTGCCAT	50.0	55.0	57.9	58.0	332-450 bp	1.0	82.5
trnH-GUG-psbA_p4	ACAATCCACTGCCTTGATCC	CCTCTAGACCTAGCTGCCAT	50.0	55.0	57.9	58.0	334-452 bp	1.0	82.5
trnH-GUG-psbA_p5	GCTATGTTATGGGCGAACGA	CCTCTAGACCTAGCTGCCAT	50.0	55.0	58.1	58.0	383-501 bp	1.0	82.1