Hotspot

trnS-UGA

LSC · 11674-11761 · 88 bp

Back to Cynodon

Pi 0.129

MarkerSeek score 90.80

Primer available yes

Label trnS-UGA

Feature Metadata

label_name: trnS-UGA
feature_type: tRNA
region: LSC
length_bp: 88
variable_sites: 11
pi: 0.129
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.97
markerseek_score: 90.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnS-UGA_p1	TATTCCATCTAGCCGAGCCA	ATAGCGAAAGTGGACTTGCA	50.0	45.0	58.0	57.5	288-291 bp	1.0	80.0
trnS-UGA_p2	TATTCCATCTAGCCGAGCCA	TAGCGAAAGTGGACTTGCAT	50.0	45.0	58.0	57.5	287-290 bp	1.0	80.0
trnS-UGA_p3	TATTCCATCTAGCCGAGCCA	AGCGAAAGTGGACTTGCATC	50.0	50.0	58.0	58.8	286-289 bp	1.0	77.1
trnS-UGA_p4	TATTCCATCTAGCCGAGCCA	GCGAAAGTGGACTTGCATCT	50.0	50.0	58.0	58.8	285-288 bp	1.0	77.1
trnS-UGA_p5	TATTCCATCTAGCCGAGCCA	CGAAAGTGGACTTGCATCTG	50.0	50.0	58.0	57.1	284-287 bp	1.0	76.7