Hotspot

rpoB

LSC · 19293-22520 · 3228 bp

Back to Cynodon

Pi 0.00124

MarkerSeek score 56.00

Primer available yes

Label rpoB

Feature Metadata

label_name: rpoB
feature_type: gene
region: LSC
length_bp: 3228
variable_sites: 4
pi: 0.00124
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 56.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpoB_p1	GGATACGACAAGCCAAGGC	ATGGCCCGTAATAATCCAGC	57.9	50.0	58.6	57.8	204-205 bp	1.0	67.1
rpoB_p2	CCAAGGCTCCGCTGAAAAA	ATGGCCCGTAATAATCCAGC	52.6	50.0	58.7	57.8	192-193 bp	1.0	66.8
rpoB_p3	GGATACGACAAGCCAAGGC	GGCCCGTAATAATCCAGCTT	57.9	50.0	58.6	57.7	202-203 bp	1.0	66.6
rpoB_p4	GGATACGACAAGCCAAGGC	AAGACCAAAAATGGCCCGTA	57.9	45.0	58.6	57.7	214-215 bp	1.0	66.3
rpoB_p5	GGATACGACAAGCCAAGGC	AGACCAAAAATGGCCCGTAA	57.9	45.0	58.6	57.7	213-214 bp	1.0	66.3