Hotspot

psbD

LSC · 9090-10151 · 1062 bp

Back to Cynodon

Pi 0.00943

MarkerSeek score 64.30

Primer available yes

Label psbD

Feature Metadata

label_name: psbD
feature_type: gene
region: LSC
length_bp: 1062
variable_sites: 10
pi: 0.00943
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 64.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbD_p1	TGCTCGGAAATGGTTGAAGT	GTCACGACCAGCTAAAACGA	45.0	50.0	57.7	58.2	1129-1130 bp	1.0	76.8
psbD_p2	TGCTCGGAAATGGTTGAAGT	TCACGACCAGCTAAAACGAA	45.0	45.0	57.7	57.5	1128-1129 bp	1.0	74.5
psbD_p3	GTGCTCGGAAATGGTTGAAG	GTCACGACCAGCTAAAACGA	50.0	50.0	57.4	58.2	1130-1131 bp	1.0	74.0
psbD_p4	TATGAGGTGCTCGGAAATGG	GTCACGACCAGCTAAAACGA	50.0	50.0	57.4	58.2	1136-1137 bp	1.0	74.0
psbD_p5	GGTGCTCGGAAATGGTTGAA	GTCACGACCAGCTAAAACGA	50.0	50.0	58.8	58.2	1131-1132 bp	1.0	72.9