Hotspot

ndhC-trnV-UAC

LSC · 49916-50865 · 950 bp

Back to Cynodon

Pi 0.00211
MarkerSeek score 60.00
Primer available yes
Label ndhC-trnV-UAC

Feature Metadata

label_name
ndhC-trnV-UAC
feature_type
igs
region
LSC
length_bp
950
variable_sites
2
pi
0.00211
unique_haplotype_count
2
primer_available
yes
alignment_reliability
1.00
markerseek_score
60.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
ndhC-trnV-UAC_p1 GGTCCTTCATTAATCGGGGC GGTTCGAATCCGTATAGCCC 55.0 55.0 58.4 57.9 1085 bp 1.0 76.4
ndhC-trnV-UAC_p2 GGTCCTTCATTAATCGGGGC AGCCCAATGCAGAAAAATGC 55.0 45.0 58.4 57.9 1178 bp 1.0 76.2
ndhC-trnV-UAC_p3 GGTCCTTCATTAATCGGGGC GGGTCTTTGAAACAGCTCGA 55.0 50.0 58.4 58.1 1153 bp 1.0 76.0
ndhC-trnV-UAC_p4 GGTCCTTCATTAATCGGGGC CGGTTCGAATCCGTATAGCC 55.0 55.0 58.4 58.3 1086 bp 1.0 74.6
ndhC-trnV-UAC_p5 GTCCTTCATTAATCGGGGCT GGTTCGAATCCGTATAGCCC 50.0 55.0 57.4 57.9 1084 bp 1.0 74.4