Hotspot

matK-trnK-UUU

LSC · 3213-3883 · 671 bp

Back to Cynodon

Pi 0.00298
MarkerSeek score 60.50
Primer available yes
Label matK-trnK-UUU

Feature Metadata

label_name
matK-trnK-UUU
feature_type
igs
region
LSC
length_bp
671
variable_sites
2
pi
0.00298
unique_haplotype_count
2
primer_available
yes
alignment_reliability
1.00
markerseek_score
60.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
matK-trnK-UUU_p1 CATAATCGTGGGCAAATGCA CAGGAGCGTTCGGTGTAATT 45.0 50.0 57.1 58.3 880 bp 1.0 71.0
matK-trnK-UUU_p2 ACATAATCGTGGGCAAATGC CAGGAGCGTTCGGTGTAATT 45.0 50.0 57.1 58.3 881 bp 1.0 71.0
matK-trnK-UUU_p3 CATAATCGTGGGCAAATGCA AGGAGCGTTCGGTGTAATTT 45.0 45.0 57.1 57.5 879 bp 1.0 69.5
matK-trnK-UUU_p4 ACATAATCGTGGGCAAATGC AGGAGCGTTCGGTGTAATTT 45.0 45.0 57.1 57.5 880 bp 1.0 69.5
matK-trnK-UUU_p5 CATAATCGTGGGCAAATGCAT CAGGAGCGTTCGGTGTAATT 42.9 50.0 57.6 58.3 880 bp 1.0 67.0