Hotspot

ycf1

SSC · 108188-113485 · 5298 bp

Back to Cynara

Pi 0.00306
MarkerSeek score 39.10
Primer available yes
Label ycf1

Feature Metadata

label_name
ycf1
feature_type
gene
region
SSC
length_bp
5298
variable_sites
30
pi
0.00306
unique_haplotype_count
4
primer_available
yes
alignment_reliability
1.00
markerseek_score
39.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
ycf1_p1 AGCCCATCAAATTCAAAACA AGGTTAAGCAGGAGACCGTA 35.0 50.0 53.9 57.8 197-203 bp 1.0 50.3
ycf1_p2 AGCCCATCAAATTCAAAACAA AGGTTAAGCAGGAGACCGTA 33.3 50.0 54.8 57.8 197-203 bp 1.0 49.3
ycf1_p3 AGCCCATCAAATTCAAAACA CTCAGGTTAAGCAGGAGACC 35.0 55.0 53.9 57.3 200-206 bp 1.0 46.6
ycf1_p4 AGCCCATCAAATTCAAAACAAT AGGTTAAGCAGGAGACCGTA 31.8 50.0 55.4 57.8 197-203 bp 1.0 46.0
ycf1_p5 AGCCCATCAAATTCAAAACAA CTCAGGTTAAGCAGGAGACC 33.3 55.0 54.8 57.3 200-206 bp 1.0 45.6