Hotspot

rpoA

LSC · 77382-78389 · 1008 bp

Pi 0.000794

MarkerSeek score 48.10

Primer available yes

Label rpoA

Feature Metadata

label_name: rpoA
feature_type: gene
region: LSC
length_bp: 1008
variable_sites: 2
pi: 0.000794
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 48.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpoA_p1	TGAATCTATGCCGATTCCGA	CATAATGGCTGTAGACCCCC	45.0	55.0	56.5	57.4	1226 bp	1.0	63.7
rpoA_p2	TGAATCTATGCCGATTCCGAA	CATAATGGCTGTAGACCCCC	42.9	55.0	57.3	57.4	1226 bp	1.0	61.6
rpoA_p3	TGAATCTATGCCGATTCCGA	ATAATGGCTGTAGACCCCCG	45.0	55.0	56.5	58.9	1225 bp	1.0	60.7
rpoA_p4	ATGAATCTATGCCGATTCCGA	CATAATGGCTGTAGACCCCC	42.9	55.0	57.0	57.4	1227 bp	1.0	59.9
rpoA_p5	TGAATCTATGCCGATTCCGAA	ATAATGGCTGTAGACCCCCG	42.9	55.0	57.3	58.9	1225 bp	1.0	58.6