Hotspot

ndhA

SSC · 115453-117604 · 2152 bp

Pi 0.00204

MarkerSeek score 43.90

Primer available yes

Label ndhA

Feature Metadata

label_name: ndhA
feature_type: gene
region: SSC
length_bp: 2152
variable_sites: 9
pi: 0.00204
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 43.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhA_p1	TGGGAGAAGTTGATCGTTGA	ACCATAGGAAGCATATCGTGA	45.0	42.9	56.5	56.3	2264-2265 bp	1.0	46.8
ndhA_p2	TGGGAGAAGTTGATCGTTGA	ACTCAGTTACCATAGGAAGCA	45.0	42.9	56.5	56.1	2272-2273 bp	1.0	44.9
ndhA_p3	TGGGAGAAGTTGATCGTTGAA	ACCATAGGAAGCATATCGTGA	42.9	42.9	57.2	56.3	2264-2265 bp	1.0	44.8
ndhA_p4	TGGGAGAAGTTGATCGTTGA	ACCATAGGAAGCATATCGTGAA	45.0	40.9	56.5	57.0	2264-2265 bp	1.0	44.6
ndhA_p5	TGGGAGAAGTTGATCGTTGA	ACACAAGTCGCGAATATAGAAGT	45.0	39.1	56.5	58.0	2201-2202 bp	1.0	44.2