Hotspot

rps12-clpP

LSC · 69303-69457 · 155 bp

Back to Cynara

Pi 0.00516
MarkerSeek score 44.60
Primer available yes
Label rps12-clpP

Feature Metadata

label_name
rps12-clpP
feature_type
igs
region
LSC
length_bp
155
variable_sites
2
pi
0.00516
unique_haplotype_count
3
primer_available
yes
alignment_reliability
1.00
markerseek_score
44.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
rps12-clpP_p1 GTACATGTTCCTCGACGCTG TTATGTCAGCAACAGAGGCC 55.0 50.0 58.7 57.9 318 bp 1.0 73.7
rps12-clpP_p2 GTACATGTTCCTCGACGCTG AACAGAGGCCCAAGCTTATG 55.0 50.0 58.7 57.9 308 bp 1.0 73.6
rps12-clpP_p3 GTACATGTTCCTCGACGCTG CAACAGAGGCCCAAGCTTAT 55.0 50.0 58.7 57.9 309 bp 1.0 73.6
rps12-clpP_p4 AGTACATGTTCCTCGACGCT TTATGTCAGCAACAGAGGCC 50.0 50.0 58.8 57.9 319 bp 1.0 73.0
rps12-clpP_p5 TACATGTTCCTCGACGCTGA TTATGTCAGCAACAGAGGCC 50.0 50.0 58.8 57.9 317 bp 1.0 73.0