Hotspot

petN-psbM

LSC · 10033-10537 · 505 bp

Back to Cynara

Pi 0.00158
MarkerSeek score 46.70
Primer available yes
Label petN-psbM

Feature Metadata

label_name
petN-psbM
feature_type
igs
region
LSC
length_bp
505
variable_sites
2
pi
0.00158
unique_haplotype_count
3
primer_available
yes
alignment_reliability
1.00
markerseek_score
46.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
petN-psbM_p1 ACATTTTCCCTTTCGCTCGT TGCTACTGCACTCTTCATTT 45.0 40.0 58.1 55.0 629 bp 1.0 55.2
petN-psbM_p2 CCCTTTCGCTCGTAGTATGG TGCTACTGCACTCTTCATTT 55.0 40.0 57.9 55.0 622 bp 1.0 55.0
petN-psbM_p3 CCTTTCGCTCGTAGTATGGG TGCTACTGCACTCTTCATTT 55.0 40.0 57.9 55.0 621 bp 1.0 55.0
petN-psbM_p4 CTTTCGCTCGTAGTATGGGG TGCTACTGCACTCTTCATTT 55.0 40.0 57.9 55.0 620 bp 1.0 55.0
petN-psbM_p5 TAGTAAGTCTCGCTTGGGCT TGCTACTGCACTCTTCATTT 50.0 40.0 58.2 55.0 667 bp 1.0 54.9