Hotspot

rpl20-rps12

LSC · 74189-75335 · 1147 bp

Back to Cynanchum

Pi 0.0536
MarkerSeek score 81.00
Primer available yes
Label rpl20-rps12

Feature Metadata

label_name
rpl20-rps12
feature_type
igs
region
LSC
length_bp
1147
variable_sites
137
pi
0.0536
unique_haplotype_count
8
primer_available
yes
alignment_reliability
0.86
markerseek_score
81.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
rpl20-rps12_p1 ACGAGCTATATATCCCCGTCT ACATGTACTCGGGTGTAT 47.6 44.4 57.4 52.1 1000-1215 bp 1.0 52.0
rpl20-rps12_p2 ACGAGCTATATATCCCCGTC ACATGTACTCGGGTGTAT 50.0 44.4 55.8 52.1 1000-1215 bp 1.0 52.0
rpl20-rps12_p3 CGAGCTATATATCCCCGTCT ACATGTACTCGGGTGTAT 50.0 44.4 55.5 52.1 999-1214 bp 1.0 52.0
rpl20-rps12_p4 ACGAGCTATATATCCCCGTCTA ACATGTACTCGGGTGTAT 45.5 44.4 57.2 52.1 1000-1215 bp 1.0 52.0
rpl20-rps12_p5 TACGAGCTATATATCCCCGTCT ACATGTACTCGGGTGTAT 45.5 44.4 57.2 52.1 1001-1216 bp 1.0 52.0