Hotspot

rpoB-trnC-GCA

LSC · 30345-31491 · 1147 bp

Back to Cynanchum

Pi 0.034

MarkerSeek score 76.70

Primer available yes

Label rpoB-trnC-GCA

Feature Metadata

label_name: rpoB-trnC-GCA
feature_type: igs
region: LSC
length_bp: 1147
variable_sites: 98
pi: 0.034
unique_haplotype_count: 8
primer_available: yes
alignment_reliability: 0.94
markerseek_score: 76.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpoB-trnC-GCA_p1	CTCATTTCCATCCACGAGCA	CCGGATTTGAACTGGGGAAA	50.0	50.0	58.0	58.1	1230-1351 bp	1.0	89.2
rpoB-trnC-GCA_p2	CCCTCATTTCCATCCACGAG	CCGGATTTGAACTGGGGAAA	55.0	50.0	58.0	58.1	1232-1353 bp	1.0	89.1
rpoB-trnC-GCA_p3	CTCATTTCCATCCACGAGCA	AAAAGGATTTGCAGTCCCCC	50.0	50.0	58.0	58.4	1212-1333 bp	1.0	87.1
rpoB-trnC-GCA_p4	CCCTCATTTCCATCCACGAG	AAAAGGATTTGCAGTCCCCC	55.0	50.0	58.0	58.4	1214-1335 bp	1.0	87.0
rpoB-trnC-GCA_p5	TTCCCTCATTTCCATCCACG	CCGGATTTGAACTGGGGAAA	50.0	50.0	57.6	58.1	1234-1355 bp	1.0	86.1