Hotspot

trnQ-UUG-psbK

LSC · 7123-7788 · 666 bp

Back to Cynanchum

Pi 0.104

MarkerSeek score 81.00

Primer available yes

Label trnQ-UUG-psbK

Feature Metadata

label_name: trnQ-UUG-psbK
feature_type: igs
region: LSC
length_bp: 666
variable_sites: 138
pi: 0.104
unique_haplotype_count: 8
primer_available: yes
alignment_reliability: 0.74
markerseek_score: 81.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnQ-UUG-psbK_p1	GACCAAAACCCGTTGCCTTA	AGGCAGAATTAAGACAGATACAGA	50.0	37.5	58.7	57.2	553-750 bp	1.0	52.0
trnQ-UUG-psbK_p2	GACCAAAACCCGTTGCCTTA	GGGCAATTTTCCGAAGAAA	50.0	42.1	58.7	54.2	588-794 bp	1.0	52.0
trnQ-UUG-psbK_p3	AAAACCCGTTGCCTTACCAC	AGGCAGAATTAAGACAGATACAGA	50.0	37.5	59.0	57.2	549-746 bp	1.0	52.0
trnQ-UUG-psbK_p4	ACCAAAACCCGTTGCCTTAC	AGGCAGAATTAAGACAGATACAGA	50.0	37.5	59.0	57.2	552-749 bp	1.0	52.0
trnQ-UUG-psbK_p5	CAAAACCCGTTGCCTTACCA	AGGCAGAATTAAGACAGATACAGA	50.0	37.5	59.0	57.2	550-747 bp	1.0	52.0