Hotspot

trnK-UUU-rps16

LSC · 4165-4883 · 719 bp

Back to Cynanchum

Pi 0.0399
MarkerSeek score 80.50
Primer available yes
Label trnK-UUU-rps16

Feature Metadata

label_name
trnK-UUU-rps16
feature_type
igs
region
LSC
length_bp
719
variable_sites
70
pi
0.0399
unique_haplotype_count
8
primer_available
yes
alignment_reliability
0.97
markerseek_score
80.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnK-UUU-rps16_p1 AAAGCCGAGTACTCTACCGT ATCCTAATCAAACGAAATTCAA 50.0 27.3 58.2 52.1 725-891 bp 1.0 50.9
trnK-UUU-rps16_p2 AAAGCCGAGTACTCTACCGT TCCTAATCAAACGAAATTCAAT 50.0 27.3 58.2 52.1 724-890 bp 1.0 50.9
trnK-UUU-rps16_p3 AAGCCGAGTACTCTACCGTT ATCCTAATCAAACGAAATTCAA 50.0 27.3 58.2 52.1 724-890 bp 1.0 50.9
trnK-UUU-rps16_p4 AAGCCGAGTACTCTACCGTT TCCTAATCAAACGAAATTCAAT 50.0 27.3 58.2 52.1 723-889 bp 1.0 50.9
trnK-UUU-rps16_p5 AAGCCGAGTACTCTACCGTT TCCTAATCAAACGAAATTCAATT 50.0 26.1 58.2 53.0 723-889 bp 1.0 50.9