Hotspot

ccsA

SSC · 111166-112131 · 966 bp

Back to Cymbopogon

Pi 0.00327

MarkerSeek score 35.40

Primer available yes

Label ccsA

Feature Metadata

label_name: ccsA
feature_type: gene
region: SSC
length_bp: 966
variable_sites: 9
pi: 0.00327
unique_haplotype_count: 6
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 35.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ccsA_p1	CTCAAGCATCTCGGTTCGAA	GGGAATGAGCGGTTTTGTTG	50.0	50.0	58.0	57.9	1587-1596 bp	1.0	80.9
ccsA_p2	AAGCATCTCGGTTCGAATCC	GGGAATGAGCGGTTTTGTTG	50.0	50.0	58.1	57.9	1584-1593 bp	1.0	80.4
ccsA_p3	CTCAAGCATCTCGGTTCGAA	GAATGAGCGGTTTTGTTGCA	50.0	45.0	58.0	57.9	1585-1594 bp	1.0	80.3
ccsA_p4	CTCAAGCATCTCGGTTCGAA	AATGAGCGGTTTTGTTGCAG	50.0	45.0	58.0	57.9	1584-1593 bp	1.0	80.3
ccsA_p5	TAGACACGCTGCTCTTAGGA	GGGAATGAGCGGTTTTGTTG	50.0	50.0	57.9	57.9	1614-1623 bp	1.0	79.9