Hotspot

trnV-GAC-rps12

IRa · 130123-131878 · 1756 bp

Back to Cymbopogon

Pi 0.000456
MarkerSeek score 50.80
Primer available yes
Label trnV-GAC-rps12

Feature Metadata

label_name
trnV-GAC-rps12
feature_type
igs
region
IRa
length_bp
1756
variable_sites
4
pi
0.000456
unique_haplotype_count
6
primer_available
yes
alignment_reliability
1.00
markerseek_score
50.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnV-GAC-rps12_p1 GATGACTTCCACCACGTCAA CGTAAGGCAGAGTTGGGTTT 50.0 50.0 57.8 58.1 1830-1841 bp 1.0 78.9
trnV-GAC-rps12_p2 AACTGATGACTTCCACCACG CGTAAGGCAGAGTTGGGTTT 50.0 50.0 57.8 58.1 1834-1845 bp 1.0 78.9
trnV-GAC-rps12_p3 ATGACTTCCACCACGTCAAG CGTAAGGCAGAGTTGGGTTT 50.0 50.0 57.8 58.1 1829-1840 bp 1.0 78.9
trnV-GAC-rps12_p4 TGGTGGGGGAGTAAGTCAAA CGTAAGGCAGAGTTGGGTTT 50.0 50.0 58.2 58.1 1895-1906 bp 1.0 78.8
trnV-GAC-rps12_p5 CTGATGACTTCCACCACGTC CGTAAGGCAGAGTTGGGTTT 55.0 50.0 58.3 58.1 1832-1843 bp 1.0 77.9