Hotspot

atpB-rbcL

LSC · 58275-59070 · 796 bp

Back to Cymbopogon

Pi 0.00251

MarkerSeek score 51.60

Primer available yes

Label atpB-rbcL

Feature Metadata

label_name: atpB-rbcL
feature_type: igs
region: LSC
length_bp: 796
variable_sites: 10
pi: 0.00251
unique_haplotype_count: 5
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 51.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
atpB-rbcL_p1	CAATTGTGGAAACCCCTGGA	ACTCCGGGGTGTAGTAAGTC	50.0	55.0	58.0	58.2	925-928 bp	0.9	75.1
atpB-rbcL_p2	TCAATTGTGGAAACCCCTGG	ACTCCGGGGTGTAGTAAGTC	50.0	55.0	58.0	58.2	926-929 bp	0.9	75.1
atpB-rbcL_p3	CAATTGTGGAAACCCCTGGA	CTCCGGGGTGTAGTAAGTCA	50.0	55.0	58.0	58.2	924-927 bp	0.9	75.1
atpB-rbcL_p4	TCAATTGTGGAAACCCCTGG	CTCCGGGGTGTAGTAAGTCA	50.0	55.0	58.0	58.2	925-928 bp	0.9	75.1
atpB-rbcL_p5	AATTGTGGAAACCCCTGGAC	ACTCCGGGGTGTAGTAAGTC	50.0	55.0	58.0	58.2	924-927 bp	0.9	75.1