Hotspot

matK-rps16

LSC · 6120-7407 · 1288 bp

Back to Cymbopogon

Pi 0.00205

MarkerSeek score 62.00

Primer available yes

Label matK-rps16

Feature Metadata

label_name: matK-rps16
feature_type: igs
region: LSC
length_bp: 1288
variable_sites: 10
pi: 0.00205
unique_haplotype_count: 9
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 62.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
matK-rps16_p1	ACGAAGTAGTGTTGACGAGA	GGTGCTCAACCTACAAGAACA	45.0	47.6	56.3	58.2	1383-1425 bp	1.0	60.7
matK-rps16_p2	TGAAAGAGAAGTGGGTAGACG	GGTGCTCAACCTACAAGAACA	47.6	47.6	57.1	58.2	1401-1443 bp	1.0	58.9
matK-rps16_p3	AGAGAAGTGGGTAGACGAAG	GGTGCTCAACCTACAAGAACA	50.0	47.6	56.0	58.2	1397-1439 bp	1.0	58.5
matK-rps16_p4	TTCCTGAAAGAGAAGTGGGT	GGTGCTCAACCTACAAGAACA	45.0	47.6	56.0	58.2	1405-1447 bp	1.0	58.2
matK-rps16_p5	TCCTGAAAGAGAAGTGGGTAGA	GGTGCTCAACCTACAAGAACA	45.5	47.6	57.9	58.2	1404-1446 bp	1.0	57.4