Hotspot

ycf1

SSC · 124021-124551 · 531 bp

Back to Cymbidium

Pi 0.0614

MarkerSeek score 72.40

Primer available yes

Label ycf1

Feature Metadata

label_name: ycf1
feature_type: gene
region: SSC
length_bp: 531
variable_sites: 167
pi: 0.0614
unique_haplotype_count: 30
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 72.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ycf1_p1	AACGAGTTTTTGGTTCCCGA	GAGCTACTGAGGAACAACGG	45.0	55.0	58.0	58.0	1768 bp	0.022727	40.6
ycf1_p2	ACGAGTTTTTGGTTCCCGAA	GAGCTACTGAGGAACAACGG	45.0	55.0	58.0	58.0	1767 bp	0.022727	40.6
ycf1_p3	AACGAGTTTTTGGTTCCCGA	GCTTCCTTCGTAACTCCAGG	45.0	55.0	58.0	58.0	1688 bp	0.022727	40.5
ycf1_p4	ACGAGTTTTTGGTTCCCGAA	GCTTCCTTCGTAACTCCAGG	45.0	55.0	58.0	58.0	1687 bp	0.022727	40.5
ycf1_p5	ACGAGTTTTTGGTTCCCGAA	AAGCGAGCCCTTTCATTCAA	45.0	45.0	58.0	58.1	1878 bp	0.022727	39.9