Hotspot

rpl14-rpl16

LSC · 82434-82573 · 140 bp

Back to Cymbidium

Pi 0.0992

MarkerSeek score 76.20

Primer available yes

Label rpl14-rpl16

Feature Metadata

label_name: rpl14-rpl16
feature_type: igs
region: LSC
length_bp: 140
variable_sites: 47
pi: 0.0992
unique_haplotype_count: 39
primer_available: yes
alignment_reliability: 0.96
markerseek_score: 76.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpl14-rpl16_p1	TCAAAAGGGTCTGAGGTTGA	GCATGCAAAATGCCTATACGA	45.0	42.9	56.3	57.4	202-211 bp	1.0	65.3
rpl14-rpl16_p2	TCAAAAGGGTCTGAGGTTGA	AGCATGCAAAATGCCTATACG	45.0	42.9	56.3	57.4	203-212 bp	1.0	65.2
rpl14-rpl16_p3	TCAAAAGGGTCTGAGGTTGAA	GCATGCAAAATGCCTATACGA	42.9	42.9	57.1	57.4	202-211 bp	1.0	63.5
rpl14-rpl16_p4	ACATTCAAAAGGGTCTGAGGT	GCATGCAAAATGCCTATACGA	42.9	42.9	57.1	57.4	206-215 bp	0.954545	62.1
rpl14-rpl16_p5	ACATTCAAAAGGGTCTGAGGT	AGCATGCAAAATGCCTATACG	42.9	42.9	57.1	57.4	207-216 bp	0.954545	62.1