Hotspot

atpB-rbcL

LSC · 55306-56246 · 941 bp

Back to Cymbidium

Pi 0.0375

MarkerSeek score 71.90

Primer available yes

Label atpB-rbcL

Feature Metadata

label_name: atpB-rbcL
feature_type: igs
region: LSC
length_bp: 941
variable_sites: 294
pi: 0.0375
unique_haplotype_count: 35
primer_available: yes
alignment_reliability: 0.97
markerseek_score: 71.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
atpB-rbcL_p1	TTTTTCCTCCACGGCGATAG	AGAAACGATCTCTCCAACGC	50.0	50.0	58.0	58.0	2119-2412 bp	1.0	91.8
atpB-rbcL_p2	TTTTTCCTCCACGGCGATAG	GTCCACACAGTTGTCCATGT	50.0	50.0	58.0	58.0	1676-1969 bp	1.0	91.6
atpB-rbcL_p3	TTTTTCCTCCACGGCGATAG	CTTTAAAAAGCGCTTCGGCA	50.0	45.0	58.0	58.0	2146-2439 bp	1.0	91.5
atpB-rbcL_p4	CTATTTTTCCTCCACGGCGA	AGAAACGATCTCTCCAACGC	50.0	50.0	58.0	58.0	2122-2415 bp	0.568182	74.3
atpB-rbcL_p5	CTATTTTTCCTCCACGGCGA	GTCCACACAGTTGTCCATGT	50.0	50.0	58.0	58.0	1679-1972 bp	0.568182	74.3