Hotspot

ndhC-trnV-UAC

LSC · 51667-52316 · 650 bp

Back to Cymbidium

Pi 0.0331

MarkerSeek score 71.30

Primer available yes

Label ndhC-trnV-UAC

Feature Metadata

label_name: ndhC-trnV-UAC
feature_type: igs
region: LSC
length_bp: 650
variable_sites: 155
pi: 0.0331
unique_haplotype_count: 35
primer_available: yes
alignment_reliability: 0.93
markerseek_score: 71.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhC-trnV-UAC_p1	CCCGAAATGACAAATGCCAA	AGAAGGTCTACGGTTCGAGT	45.0	50.0	57.3	57.8	653-782 bp	1.0	84.5
ndhC-trnV-UAC_p2	TCCCCGAAATGACAAATGCC	AGAAGGTCTACGGTTCGAGT	50.0	50.0	58.8	57.8	655-784 bp	1.0	83.9
ndhC-trnV-UAC_p3	CCCGAAATGACAAATGCCAA	GAAGGTCTACGGTTCGAGTC	45.0	55.0	57.3	57.5	652-781 bp	1.0	81.8
ndhC-trnV-UAC_p4	CCCCGAAATGACAAATGCCA	AGAAGGTCTACGGTTCGAGT	50.0	50.0	59.1	57.8	654-783 bp	1.0	81.6
ndhC-trnV-UAC_p5	TCCCCGAAATGACAAATGCC	GAAGGTCTACGGTTCGAGTC	50.0	55.0	58.8	57.5	654-783 bp	1.0	81.1