Hotspot

rps16-trnQ-UUG

LSC · 6643-7590 · 948 bp

Back to Cymbidium

Pi 0.031

MarkerSeek score 73.10

Primer available yes

Label rps16-trnQ-UUG

Feature Metadata

label_name: rps16-trnQ-UUG
feature_type: igs
region: LSC
length_bp: 948
variable_sites: 233
pi: 0.031
unique_haplotype_count: 33
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 73.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rps16-trnQ-UUG_p1	AAGTCGCACGTTGCTTTCTA	ACCAACTAAAAATGGGGCGT	45.0	45.0	58.1	58.0	929-1158 bp	1.0	90.9
rps16-trnQ-UUG_p2	TCATGTCCTTCAAGTCGCAC	ACCAACTAAAAATGGGGCGT	50.0	45.0	58.2	58.0	940-1169 bp	1.0	90.3
rps16-trnQ-UUG_p3	AAGTCGCACGTTGCTTTCTA	GAGGTTCGAATCCTTCCGTC	45.0	55.0	58.1	58.1	869-1098 bp	1.0	90.3
rps16-trnQ-UUG_p4	TCATGTCCTTCAAGTCGCAC	GAGGTTCGAATCCTTCCGTC	50.0	55.0	58.2	58.1	880-1109 bp	1.0	89.8
rps16-trnQ-UUG_p5	AAGTCGCACGTTGCTTTCTA	CAACTAAAAATGGGGCGTGG	45.0	50.0	58.1	57.6	927-1156 bp	1.0	88.0