primer_id	label_name	rank	fwd_seq	rev_seq	fwd_len	rev_len	fwd_gc	rev_gc	fwd_tm	rev_tm	fwd_self_any_th	rev_self_any_th	primer3_penalty	target_start	target_end	amplicon_size	cross_species_success_rate	primer_score
trnK-UUU-rps16_p1	trnK-UUU-rps16	1	AAAGCCGAGTACTCTACCGT	AGAAAGGAAAAACAAGAATCATGA	20	24	50.0	29.2	58.2	55.2	20.3	0.0	6.971417	4948	5499	521-799	0.931818	49.3
trnK-UUU-rps16_p2	trnK-UUU-rps16	2	AAGCCGAGTACTCTACCGTT	AGAAAGGAAAAACAAGAATCATGA	20	24	50.0	29.2	58.2	55.2	20.3	0.0	6.971417	4948	5499	520-798	0.931818	49.3
trnK-UUU-rps16_p3	trnK-UUU-rps16	3	TACCGTTGAGTTAGCAACCC	AGAAAGGAAAAACAAGAATCATGA	20	24	50.0	29.2	57.5	55.2	0.0	0.0	7.263754	4948	5499	507-785	0.931818	49.3
trnK-UUU-rps16_p4	trnK-UUU-rps16	4	AAAGCCGAGTACTCTACCGT	AAGAAAGGAAAAACAAGAATCATGA	20	25	50.0	28.0	58.2	55.9	20.3	0.0	7.282181	4948	5499	522-800	0.931818	49.3
trnK-UUU-rps16_p5	trnK-UUU-rps16	5	AAGCCGAGTACTCTACCGTT	AAGAAAGGAAAAACAAGAATCATGA	20	25	50.0	28.0	58.2	55.9	20.3	0.0	7.282181	4948	5499	521-799	0.931818	49.3
rps16-trnQ-UUG_p1	rps16-trnQ-UUG	1	AAGTCGCACGTTGCTTTCTA	ACCAACTAAAAATGGGGCGT	20	20	45.0	45.0	58.1	58.0	8.1	0.0	0.143261	6643	7590	929-1158	1.000000	90.9
rps16-trnQ-UUG_p2	rps16-trnQ-UUG	2	TCATGTCCTTCAAGTCGCAC	ACCAACTAAAAATGGGGCGT	20	20	50.0	45.0	58.2	58.0	0.0	0.0	0.208169	6643	7590	940-1169	1.000000	90.3
rps16-trnQ-UUG_p3	rps16-trnQ-UUG	3	AAGTCGCACGTTGCTTTCTA	GAGGTTCGAATCCTTCCGTC	20	20	45.0	55.0	58.1	58.1	8.1	16.9	0.215425	6643	7590	869-1098	1.000000	90.3
rps16-trnQ-UUG_p4	rps16-trnQ-UUG	4	TCATGTCCTTCAAGTCGCAC	GAGGTTCGAATCCTTCCGTC	20	20	50.0	55.0	58.2	58.1	0.0	16.9	0.280333	6643	7590	880-1109	1.000000	89.8
rps16-trnQ-UUG_p5	rps16-trnQ-UUG	5	AAGTCGCACGTTGCTTTCTA	CAACTAAAAATGGGGCGTGG	20	20	45.0	50.0	58.1	57.6	8.1	0.0	0.501493	6643	7590	927-1156	1.000000	88.0
trnS-GCU-trnR-UCU_p1	trnS-GCU-trnR-UCU	1	CAATCCGACGCTTTAGTCCA	AGAAGACCTCTGTCCTATCCA	20	21	50.0	47.6	58.0	56.9	0.0	0.0	2.056187	9010	10553	1530-1739	1.000000	75.6
trnS-GCU-trnR-UCU_p2	trnS-GCU-trnR-UCU	2	AATCCGACGCTTTAGTCCAC	AGAAGACCTCTGTCCTATCCA	20	21	50.0	47.6	58.0	56.9	0.0	0.0	2.057575	9010	10553	1529-1738	1.000000	75.5
trnS-GCU-trnR-UCU_p3	trnS-GCU-trnR-UCU	3	CAATCCGACGCTTTAGTCCA	AGGTTTAGAAGACCTCTGTCCT	20	22	50.0	45.5	58.0	57.9	0.0	0.0	2.139515	9010	10553	1536-1745	1.000000	74.9
trnS-GCU-trnR-UCU_p4	trnS-GCU-trnR-UCU	4	AATCCGACGCTTTAGTCCAC	AGGTTTAGAAGACCTCTGTCCT	20	22	50.0	45.5	58.0	57.9	0.0	0.0	2.140903	9010	10553	1535-1744	1.000000	74.9
trnS-GCU-trnR-UCU_p5	trnS-GCU-trnR-UCU	5	AGCAATCCGACGCTTTAGTC	AGAAGACCTCTGTCCTATCCA	20	21	50.0	47.6	58.4	56.9	0.0	0.0	2.410028	9010	10553	1532-1741	1.000000	72.7
atpH-atpI_p1	atpH-atpI	1	CGCAATACCTTCTACGGCTT	AACTTTGGCTGCGGCTTATA	20	20	50.0	45.0	58.1	57.9	0.0	18.8	0.186851	14256	14839	706-830	1.000000	89.7
atpH-atpI_p2	atpH-atpI	2	TCCGATAGAAGCAAGCCCTA	AACTTTGGCTGCGGCTTATA	20	20	50.0	45.0	57.9	57.9	0.0	18.8	0.204923	14256	14839	655-779	1.000000	89.7
atpH-atpI_p3	atpH-atpI	3	AGCCAATCCAGCAGCAATAA	AACTTTGGCTGCGGCTTATA	20	20	45.0	45.0	57.9	57.9	0.0	18.8	0.271229	14256	14839	634-758	1.000000	89.3
atpH-atpI_p4	atpH-atpI	4	CCGATAGAAGCAAGCCCTAC	AACTTTGGCTGCGGCTTATA	20	20	55.0	45.0	57.8	57.9	0.0	18.8	0.279126	14256	14839	654-778	1.000000	89.0
atpH-atpI_p5	atpH-atpI	5	GCCAATCCAGCAGCAATAAC	AACTTTGGCTGCGGCTTATA	20	20	50.0	45.0	57.8	57.9	0.0	18.8	0.337170	14256	14839	633-757	1.000000	88.8
rpoB-trnC-GCA_p1	rpoB-trnC-GCA	1	GTGGACATTCCCTCATTTCCA	TAAAGGATTTGCAGTCCCCC	21	20	47.6	50.0	57.9	57.5	0.0	14.6	1.611057	27216	28682	1311-1562	0.931818	76.4
rpoB-trnC-GCA_p2	rpoB-trnC-GCA	2	TGTGGACATTCCCTCATTTCC	TAAAGGATTTGCAGTCCCCC	21	20	47.6	50.0	57.9	57.5	0.0	14.6	1.611057	27216	28682	1312-1563	0.931818	76.4
rpoB-trnC-GCA_p3	rpoB-trnC-GCA	3	GTGGACATTCCCTCATTTCCA	CGACACCCAGATTCGAACTG	21	20	47.6	55.0	57.9	58.6	0.0	16.2	1.735759	27216	28682	1335-1586	0.954545	76.3
rpoB-trnC-GCA_p4	rpoB-trnC-GCA	4	TGTGGACATTCCCTCATTTCC	CGACACCCAGATTCGAACTG	21	20	47.6	55.0	57.9	58.6	0.0	16.2	1.735759	27216	28682	1336-1587	0.954545	76.3
rpoB-trnC-GCA_p5	rpoB-trnC-GCA	5	CCAGGTATTGTGGACATTCCC	TAAAGGATTTGCAGTCCCCC	21	20	52.4	50.0	58.3	57.5	0.0	14.6	1.870242	27216	28682	1151-1571	0.977273	76.1
psbM-petN_p1	psbM-petN	1	CTTGCGCTTATTGCTACTGC	TAGTAAGTCTCGCTTGGGCT	20	20	50.0	50.0	57.9	58.2	9.7	0.0	0.248147	29845	30647	612-1012	1.000000	90.0
psbM-petN_p2	psbM-petN	2	CTTGCGCTTATTGCTACTGC	AGGGGACATCATTTGCATGG	20	20	50.0	50.0	57.9	58.2	9.7	0.0	0.312441	29845	30647	635-1035	1.000000	89.5
psbM-petN_p3	psbM-petN	3	CTTGCGCTTATTGCTACTGC	GGGGACATCATTTGCATGGA	20	20	50.0	50.0	57.9	58.2	9.7	0.0	0.317578	29845	30647	634-1034	1.000000	89.5
psbM-petN_p4	psbM-petN	4	ACGTCAAAACAGTCAGCCAA	TAGTAAGTCTCGCTTGGGCT	20	20	45.0	50.0	58.3	58.2	0.0	0.0	0.406116	29845	30647	551-951	1.000000	88.8
psbM-petN_p5	psbM-petN	5	ATTGCTACTGCGCTGTTCAT	TAGTAAGTCTCGCTTGGGCT	20	20	45.0	50.0	58.3	58.2	25.3	0.0	0.408587	29845	30647	603-1003	1.000000	88.7
trnT-UGU-trnL-UAA_p1	trnT-UGU-trnL-UAA	1	GCGATGCTCTAACCTCTGAG	ACCAATTTCGCCATATCCCC	20	20	55.0	50.0	57.9	58.0	0.0	0.0	0.137364	47760	48355	616-715	1.000000	90.9
trnT-UGU-trnL-UAA_p2	trnT-UGU-trnL-UAA	2	GCGATGCTCTAACCTCTGAG	TAGCGTCTACCAATTTCGCC	20	20	55.0	50.0	57.9	58.1	0.0	0.0	0.202173	47760	48355	624-723	1.000000	90.4
trnT-UGU-trnL-UAA_p3	trnT-UGU-trnL-UAA	3	GGAATCGAACCGATGACCAT	ACCAATTTCGCCATATCCCC	20	20	50.0	50.0	57.8	58.0	15.5	0.0	0.245117	47760	48355	648-747	1.000000	90.0
trnT-UGU-trnL-UAA_p4	trnT-UGU-trnL-UAA	4	AGAAAAAGAGCCGGCTATCG	ACCAATTTCGCCATATCCCC	20	20	50.0	50.0	58.1	58.0	33.0	0.0	0.063299	47760	48355	667-766	0.931818	88.8
trnT-UGU-trnL-UAA_p5	trnT-UGU-trnL-UAA	5	AGAAAAAGAGCCGGCTATCG	TAGCGTCTACCAATTTCGCC	20	20	50.0	50.0	58.1	58.1	33.0	0.0	0.128108	47760	48355	675-774	0.931818	88.2
ndhC-trnV-UAC_p1	ndhC-trnV-UAC	1	CCCGAAATGACAAATGCCAA	AGAAGGTCTACGGTTCGAGT	20	20	45.0	50.0	57.3	57.8	0.0	0.0	0.937168	51667	52316	653-782	1.000000	84.5
ndhC-trnV-UAC_p2	ndhC-trnV-UAC	2	TCCCCGAAATGACAAATGCC	AGAAGGTCTACGGTTCGAGT	20	20	50.0	50.0	58.8	57.8	0.0	0.0	1.015497	51667	52316	655-784	1.000000	83.9
ndhC-trnV-UAC_p3	ndhC-trnV-UAC	3	CCCGAAATGACAAATGCCAA	GAAGGTCTACGGTTCGAGTC	20	20	45.0	55.0	57.3	57.5	0.0	0.0	1.280940	51667	52316	652-781	1.000000	81.8
ndhC-trnV-UAC_p4	ndhC-trnV-UAC	4	CCCCGAAATGACAAATGCCA	AGAAGGTCTACGGTTCGAGT	20	20	50.0	50.0	59.1	57.8	0.0	0.0	1.303142	51667	52316	654-783	1.000000	81.6
ndhC-trnV-UAC_p5	ndhC-trnV-UAC	5	TCCCCGAAATGACAAATGCC	GAAGGTCTACGGTTCGAGTC	20	20	50.0	55.0	58.8	57.5	0.0	0.0	1.359269	51667	52316	654-783	1.000000	81.1
atpB-rbcL_p1	atpB-rbcL	1	TTTTTCCTCCACGGCGATAG	AGAAACGATCTCTCCAACGC	20	20	50.0	50.0	58.0	58.0	16.6	0.0	0.020873	55306	56246	2119-2412	1.000000	91.8
atpB-rbcL_p2	atpB-rbcL	2	TTTTTCCTCCACGGCGATAG	GTCCACACAGTTGTCCATGT	20	20	50.0	50.0	58.0	58.0	16.6	0.0	0.051223	55306	56246	1676-1969	1.000000	91.6
atpB-rbcL_p3	atpB-rbcL	3	TTTTTCCTCCACGGCGATAG	CTTTAAAAAGCGCTTCGGCA	20	20	50.0	45.0	58.0	58.0	16.6	25.2	0.063430	55306	56246	2146-2439	1.000000	91.5
atpB-rbcL_p4	atpB-rbcL	4	CTATTTTTCCTCCACGGCGA	AGAAACGATCTCTCCAACGC	20	20	50.0	50.0	58.0	58.0	16.6	0.0	0.020873	55306	56246	2122-2415	0.568182	74.3
atpB-rbcL_p5	atpB-rbcL	5	CTATTTTTCCTCCACGGCGA	GTCCACACAGTTGTCCATGT	20	20	50.0	50.0	58.0	58.0	16.6	0.0	0.051223	55306	56246	1679-1972	0.568182	74.3
rpl14-rpl16_p1	rpl14-rpl16	1	TCAAAAGGGTCTGAGGTTGA	GCATGCAAAATGCCTATACGA	20	21	45.0	42.9	56.3	57.4	0.0	14.2	3.338403	82434	82573	202-211	1.000000	65.3
rpl14-rpl16_p2	rpl14-rpl16	2	TCAAAAGGGTCTGAGGTTGA	AGCATGCAAAATGCCTATACG	20	21	45.0	42.9	56.3	57.4	0.0	22.0	3.345223	82434	82573	203-212	1.000000	65.2
rpl14-rpl16_p3	rpl14-rpl16	3	TCAAAAGGGTCTGAGGTTGAA	GCATGCAAAATGCCTATACGA	21	21	42.9	42.9	57.1	57.4	0.0	14.2	3.567997	82434	82573	202-211	1.000000	63.5
rpl14-rpl16_p4	rpl14-rpl16	4	ACATTCAAAAGGGTCTGAGGT	GCATGCAAAATGCCTATACGA	21	21	42.9	42.9	57.1	57.4	0.0	14.2	3.508364	82434	82573	206-215	0.954545	62.1
rpl14-rpl16_p5	rpl14-rpl16	5	ACATTCAAAAGGGTCTGAGGT	AGCATGCAAAATGCCTATACG	21	21	42.9	42.9	57.1	57.4	0.0	22.0	3.515183	82434	82573	207-216	0.954545	62.1
ndhF-rpl32_p1	ndhF-rpl32	1	TATCTTCCCTGTCCCAAGCA	AAGTTTCGTTCTCAGTCGGG	20	20	50.0	50.0	58.0	57.8	0.0	0.0	0.184983	114901	115850	1928-2836	0.181818	51.6
ndhF-rpl32_p2	ndhF-rpl32	2	TCTTCCCTGTCCCAAGCATA	AAGTTTCGTTCTCAGTCGGG	20	20	50.0	50.0	58.0	57.8	0.0	0.0	0.184983	114901	115850	1926-2834	0.181818	51.6
ndhF-rpl32_p3	ndhF-rpl32	3	TATCTTCCCTGTCCCAAGCA	TTCGTTCTCAGTCGGGGTAT	20	20	50.0	50.0	58.0	58.2	0.0	0.0	0.191218	114901	115850	1924-2832	0.181818	51.6
ndhF-rpl32_p4	ndhF-rpl32	4	TCTTCCCTGTCCCAAGCATA	TTCGTTCTCAGTCGGGGTAT	20	20	50.0	50.0	58.0	58.2	0.0	0.0	0.191218	114901	115850	1922-2830	0.181818	51.6
ndhF-rpl32_p5	ndhF-rpl32	5	TCTTCCCTGTCCCAAGCATA	TTTCGTTCTCAGTCGGGGTA	20	20	50.0	50.0	58.0	58.4	0.0	0.0	0.414932	114901	115850	1923-2831	0.181818	49.8
ccsA-ndhD_p1	ccsA-ndhD	1	AGTTATGGTTCATTCCAATTACT	TCGGTTGACAAGGTACAAGT	23	20	30.4	45.0	53.7	56.7	0.0	0.0	8.590784	117900	118193	355-377	0.863636	46.5
ccsA-ndhD_p2	ccsA-ndhD	2	TGGTTCATTCCAATTACTATCT	TCGGTTGACAAGGTACAAGT	22	20	31.8	45.0	52.6	56.7	0.0	0.0	8.722491	117900	118193	350-372	0.863636	46.5
ccsA-ndhD_p3	ccsA-ndhD	3	AGTTATGGTTCATTCCAATTACT	TCGGTTGACAAGGTACAAGTT	23	21	30.4	42.9	53.7	57.5	0.0	0.0	8.853681	117900	118193	355-377	0.863636	46.5
ccsA-ndhD_p4	ccsA-ndhD	4	TGGTTCATTCCAATTACTATCT	TCGGTTGACAAGGTACAAGTT	22	21	31.8	42.9	52.6	57.5	0.0	0.0	8.985388	117900	118193	350-372	0.863636	46.5
ccsA-ndhD_p5	ccsA-ndhD	5	ATGGTTCATTCCAATTACTATCT	TCGGTTGACAAGGTACAAGT	23	20	30.4	45.0	53.2	56.7	0.0	0.0	9.067301	117900	118193	351-373	0.863636	46.5
ycf1_p1	ycf1	1	AACGAGTTTTTGGTTCCCGA	GAGCTACTGAGGAACAACGG	20	20	45.0	55.0	58.0	58.0	0.0	0.0	0.043582	124021	124551	1768-1768	0.022727	40.6
ycf1_p2	ycf1	2	ACGAGTTTTTGGTTCCCGAA	GAGCTACTGAGGAACAACGG	20	20	45.0	55.0	58.0	58.0	0.0	0.0	0.043582	124021	124551	1767-1767	0.022727	40.6
ycf1_p3	ycf1	3	AACGAGTTTTTGGTTCCCGA	GCTTCCTTCGTAACTCCAGG	20	20	45.0	55.0	58.0	58.0	0.0	0.0	0.052791	124021	124551	1688-1688	0.022727	40.5
ycf1_p4	ycf1	4	ACGAGTTTTTGGTTCCCGAA	GCTTCCTTCGTAACTCCAGG	20	20	45.0	55.0	58.0	58.0	0.0	0.0	0.052791	124021	124551	1687-1687	0.022727	40.5
ycf1_p5	ycf1	5	ACGAGTTTTTGGTTCCCGAA	AAGCGAGCCCTTTCATTCAA	20	20	45.0	45.0	58.0	58.1	0.0	0.0	0.131551	124021	124551	1878-1878	0.022727	39.9