Hotspot

ndhD

SSC · 117316-118173 · 858 bp

Back to Cymbaria

Pi 0.0527

MarkerSeek score 74.40

Primer available yes

Label ndhD

Feature Metadata

label_name: ndhD
feature_type: gene
region: SSC
length_bp: 858
variable_sites: 45
pi: 0.0527
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 74.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhD_p1	GGGAGGCCATCAAAAAGCTA	TCTGCTCCAAGGACTGAAGA	50.0	50.0	57.9	58.0	1421-1436 bp	1.0	83.4
ndhD_p2	GGGGGCCAGAATCAGAAAAA	TCTGCTCCAAGGACTGAAGA	50.0	50.0	58.1	58.0	1651 bp	0.5	59.4
ndhD_p3	GGGGGCCAGAATCAGAAAAA	TCTCGCTCATCAACCCATTG	50.0	50.0	58.1	58.0	1912 bp	0.5	59.3
ndhD_p4	GGGGGCCAGAATCAGAAAAA	CTGCTCCAAGGACTGAAGAC	50.0	55.0	58.1	57.9	1650 bp	0.5	58.8
ndhD_p5	GGGGGCCAGAATCAGAAAAA	GCTCCAAGGACTGAAGACTG	50.0	55.0	58.1	57.9	1648 bp	0.5	58.8