Hotspot

accD

LSC · 59570-61966 · 2397 bp

Back to Cymbaria

Pi 0.0415

MarkerSeek score 68.10

Primer available yes

Label accD

Feature Metadata

label_name: accD
feature_type: gene
region: LSC
length_bp: 2397
variable_sites: 85
pi: 0.0415
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.86
markerseek_score: 68.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
accD_p1	GATCTCTTACACAACGCGGT	AGATCGTTTTCTGCAGGCAT	50.0	45.0	58.0	57.9	2718-2977 bp	1.0	85.8
accD_p2	TGATCTCTTACACAACGCGG	AGATCGTTTTCTGCAGGCAT	50.0	45.0	58.0	57.9	2719-2978 bp	1.0	85.8
accD_p3	GATCTCTTACACAACGCGGT	TTGCAATGGCCGGAAATACT	50.0	45.0	58.0	58.2	2531-2785 bp	1.0	85.6
accD_p4	TGATCTCTTACACAACGCGG	TTGCAATGGCCGGAAATACT	50.0	45.0	58.0	58.2	2532-2786 bp	1.0	85.6
accD_p5	GATCTCTTACACAACGCGGT	ACCACAGATCGTTTTCTGCA	50.0	45.0	58.0	57.7	2982 bp	0.5	57.9