Hotspot

ycf2-trnM-CAU

IRa · 146952-149258 · 2307 bp

Back to Cymbaria

Pi 0.024

MarkerSeek score 75.20

Primer available yes

Label ycf2-trnM-CAU

Feature Metadata

label_name: ycf2-trnM-CAU
feature_type: igs
region: IRa
length_bp: 2307
variable_sites: 52
pi: 0.024
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.94
markerseek_score: 75.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ycf2-trnM-CAU_p1	AGACAAAGCCACATGGAGAC	GTTGGGCGCTTTAACCATTC	50.0	50.0	57.8	58.0	2244-2384 bp	1.0	84.2
ycf2-trnM-CAU_p2	GACAAAGCCACATGGAGACT	GTTGGGCGCTTTAACCATTC	50.0	50.0	57.8	58.0	2243-2383 bp	1.0	84.2
ycf2-trnM-CAU_p3	AGACAAAGCCACATGGAGAC	AGTTGGGCGCTTTAACCATT	50.0	45.0	57.8	58.4	2245-2385 bp	1.0	81.3
ycf2-trnM-CAU_p4	GACAAAGCCACATGGAGACT	AGTTGGGCGCTTTAACCATT	50.0	45.0	57.8	58.4	2244-2384 bp	1.0	81.3
ycf2-trnM-CAU_p5	GACAAAGCCACATGGAGACT	TTGGGCGCTTTAACCATTCA	50.0	45.0	57.8	58.4	2242-2382 bp	1.0	81.2