Hotspot

ycf1-rrn5S

SSC · 124832-128432 · 3601 bp

Back to Cymbaria

Pi 0.0629

MarkerSeek score 67.70

Primer available yes

Label ycf1-rrn5S

Feature Metadata

label_name: ycf1-rrn5S
feature_type: igs
region: SSC
length_bp: 3601
variable_sites: 169
pi: 0.0629
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.75
markerseek_score: 67.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ycf1-rrn5S_p1	AGCTGTCCCACAAGAAAACC	AGACTGAGAGGTCTTAAGCT	50.0	45.0	58.3	54.9	753 bp	0.5	32.7
ycf1-rrn5S_p2	AACCAGTTCCTGCTGCTATC	AGACTGAGAGGTCTTAAGCT	50.0	45.0	57.6	54.9	737 bp	0.5	31.9
ycf1-rrn5S_p3	ACCAGTTCCTGCTGCTATCT	AGACTGAGAGGTCTTAAGCT	50.0	45.0	58.4	54.9	736 bp	0.5	31.8
ycf1-rrn5S_p4	GTCTCTGTGATTGTGCCAGA	AGACTGAGAGGTCTTAAGCT	50.0	45.0	57.5	54.9	874 bp	0.5	31.4
ycf1-rrn5S_p5	AGTCTCTGTGATTGTGCCAG	AGACTGAGAGGTCTTAAGCT	50.0	45.0	57.5	54.9	875 bp	0.5	31.4