Hotspot

trnN-GUU-ndhF

SSC · 111438-113551 · 2114 bp

Back to Cymbaria

Pi 0.0572

MarkerSeek score 77.60

Primer available yes

Label trnN-GUU-ndhF

Feature Metadata

label_name: trnN-GUU-ndhF
feature_type: igs
region: SSC
length_bp: 2114
variable_sites: 117
pi: 0.0572
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.97
markerseek_score: 77.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnN-GUU-ndhF_p1	AGGATTCGAACCTACGACCA	TCAGCAAATAAATAGCAGTTCT	50.0	31.8	58.2	53.8	2207 bp	0.5	20.0
trnN-GUU-ndhF_p2	CTCTACCGCTGAGCTACTGA	TCAGCAAATAAATAGCAGTTCT	55.0	31.8	58.3	53.8	2168 bp	0.5	20.0
trnN-GUU-ndhF_p3	TCTACCGCTGAGCTACTGAG	TCAGCAAATAAATAGCAGTTCT	55.0	31.8	58.3	53.8	2167 bp	0.5	20.0
trnN-GUU-ndhF_p4	AGGATTCGAACCTACGACCA	TTCAGCAAATAAATAGCAGTTCT	50.0	30.4	58.2	54.6	2208 bp	0.5	20.0
trnN-GUU-ndhF_p5	TCTACCGCTGAGCTACTGAG	TTCAGCAAATAAATAGCAGTTCT	55.0	30.4	58.3	54.6	2168 bp	0.5	20.0