Hotspot

trnR-ACG-trnN-GUU

SSC · 110803-111365 · 563 bp

Back to Cymbaria

Pi 0.0716

MarkerSeek score 80.70

Primer available yes

Label trnR-ACG-trnN-GUU

Feature Metadata

label_name: trnR-ACG-trnN-GUU
feature_type: igs
region: SSC
length_bp: 563
variable_sites: 40
pi: 0.0716
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 80.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnR-ACG-trnN-GUU_p1	CAGAGGATTAGAGCACGTGG	TGGTCGTAGGTTCGAATCCT	55.0	50.0	57.8	58.2	655-673 bp	1.0	82.3
trnR-ACG-trnN-GUU_p2	CAGAGGATTAGAGCACGTGG	CGATTGGTCGTAGGTTCGAA	55.0	50.0	57.8	57.7	659-677 bp	1.0	81.4
trnR-ACG-trnN-GUU_p3	CAGAGGATTAGAGCACGTGG	CTCAGTAGCTCAGCGGTAGA	55.0	55.0	57.8	58.3	695-713 bp	1.0	80.6
trnR-ACG-trnN-GUU_p4	CAGAGGATTAGAGCACGTGG	TCAGTAGCTCAGCGGTAGAG	55.0	55.0	57.8	58.3	694-712 bp	1.0	80.6
trnR-ACG-trnN-GUU_p5	CAGAGGATTAGAGCACGTGG	TTGGTCGTAGGTTCGAATCC	55.0	50.0	57.8	57.3	656-674 bp	1.0	78.3