Hotspot

rrn5S-trnR-ACG

SSC · 110490-110728 · 239 bp

Back to Cymbaria

Pi 0.138
MarkerSeek score 77.70
Primer available yes
Label rrn5S-trnR-ACG

Feature Metadata

label_name
rrn5S-trnR-ACG
feature_type
igs
region
SSC
length_bp
239
variable_sites
31
pi
0.138
unique_haplotype_count
2
primer_available
yes
alignment_reliability
0.94
markerseek_score
77.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
rrn5S-trnR-ACG_p1 TCGTTCATGGTTCGACATCC GGTACTGACCTTTTTGGCCA 50.0 50.0 58.0 58.0 470 bp 0.5 59.9
rrn5S-trnR-ACG_p2 CGTTCATGGTTCGACATCCT GGTACTGACCTTTTTGGCCA 50.0 50.0 58.0 58.0 469 bp 0.5 59.8
rrn5S-trnR-ACG_p3 TCGTTCATGGTTCGACATCC TTCAGAATAAAGGGGCTCGC 50.0 50.0 58.0 58.0 987 bp 0.5 59.7
rrn5S-trnR-ACG_p4 CGTTCATGGTTCGACATCCT TTCAGAATAAAGGGGCTCGC 50.0 50.0 58.0 58.0 986 bp 0.5 59.6
rrn5S-trnR-ACG_p5 GGAAAAATAGCTCGAGGCCA GGTACTGACCTTTTTGGCCA 50.0 50.0 58.0 58.0 358 bp 0.5 59.6