Hotspot

psaJ-rpl33

LSC · 70268-70756 · 489 bp

Back to Cymbaria

Pi 0.0641

MarkerSeek score 76.50

Primer available yes

Label psaJ-rpl33

Feature Metadata

label_name: psaJ-rpl33
feature_type: igs
region: LSC
length_bp: 489
variable_sites: 30
pi: 0.0641
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.96
markerseek_score: 76.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psaJ-rpl33_p1	CGCTATGGTTCGGGGTTTTA	TGCCCCTTGGTACCTTCTTA	50.0	50.0	58.0	58.0	657-666 bp	1.0	85.7
psaJ-rpl33_p2	TCGGGGTTTTAGCAGGTCTA	TGCCCCTTGGTACCTTCTTA	50.0	50.0	58.1	58.0	648-657 bp	1.0	85.5
psaJ-rpl33_p3	CGCTATGGTTCGGGGTTTTA	ACAATCAATTCGATCCCCCG	50.0	50.0	58.0	58.0	1027-1034 bp	1.0	85.3
psaJ-rpl33_p4	TCGGGGTTTTAGCAGGTCTA	ACAATCAATTCGATCCCCCG	50.0	50.0	58.1	58.0	1018-1025 bp	1.0	85.1
psaJ-rpl33_p5	CGCTATGGTTCGGGGTTTTA	GGGACAAAGGCCTACGAAAA	50.0	50.0	58.0	58.1	998-1005 bp	1.0	84.9