Hotspot

rps16-trnK-UUU

LSC · 54253-55332 · 1080 bp

Back to Cymbaria

Pi 0.0475
MarkerSeek score 77.60
Primer available yes
Label rps16-trnK-UUU

Feature Metadata

label_name
rps16-trnK-UUU
feature_type
igs
region
LSC
length_bp
1080
variable_sites
49
pi
0.0475
unique_haplotype_count
2
primer_available
yes
alignment_reliability
0.96
markerseek_score
77.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
rps16-trnK-UUU_p1 GAACTTCCGTCTAATCAAATGA AAAGCCGAGTACTCTACCGT 36.4 50.0 54.2 58.2 1147-1151 bp 1.0 46.8
rps16-trnK-UUU_p2 GAACTTCCGTCTAATCAAATGA AAGCCGAGTACTCTACCGTT 36.4 50.0 54.2 58.2 1146-1150 bp 1.0 46.8
rps16-trnK-UUU_p3 GAACTTCCGTCTAATCAAATGA TCTAGCCGCACTTAAAAGCC 36.4 50.0 54.2 58.3 1161-1165 bp 1.0 46.7
rps16-trnK-UUU_p4 GAACTTCCGTCTAATCAAATGA TCAGTCGTGGTCTTCCAAAC 36.4 50.0 54.2 57.8 1232-1236 bp 1.0 46.3
rps16-trnK-UUU_p5 GAACTTCCGTCTAATCAAATGA CAGTCGTGGTCTTCCAAACT 36.4 50.0 54.2 57.8 1231-1235 bp 1.0 46.3