Hotspot

rps16

LSC · 4929-6048 · 1120 bp

Back to Cymatocarpus

Pi 0.019

MarkerSeek score 63.80

Primer available yes

Label rps16

Feature Metadata

label_name: rps16
feature_type: gene
region: LSC
length_bp: 1120
variable_sites: 32
pi: 0.019
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 63.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rps16_p1	GATGGAAGCGAAAGAGGAGG	ACTGACTGAACTATGACTATTCATGA	55.0	34.6	58.1	57.7	1276-1285 bp	1.0	42.6
rps16_p2	ATGGAAGCGAAAGAGGAGGA	ACGATATACTGACTGAACTATGACT	50.0	36.0	58.4	57.0	1282-1291 bp	1.0	42.6
rps16_p3	GATGGAAGCGAAAGAGGAGG	ACTGACTGAACTATGACTATTCA	55.0	34.8	58.1	54.6	1276-1285 bp	1.0	42.6
rps16_p4	GATGGAAGCGAAAGAGGAGG	ACGATATACTGACTGAACTATGACT	55.0	36.0	58.1	57.0	1283-1292 bp	1.0	42.5
rps16_p5	GATGGAAGCGAAAGAGGAGG	ACGATATACTGACTGAACTATGAC	55.0	37.5	58.1	55.7	1283-1292 bp	1.0	42.5