Hotspot

rps15-ycf1

SSC · 123629-123984 · 356 bp

Back to Cymatocarpus

Pi 0.0338

MarkerSeek score 67.40

Primer available yes

Label rps15-ycf1

Feature Metadata

label_name: rps15-ycf1
feature_type: igs
region: SSC
length_bp: 356
variable_sites: 17
pi: 0.0338
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 0.97
markerseek_score: 67.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rps15-ycf1_p1	TCTTGAAATGAAATGAATGCA	TGGTTTAATACTACTAACGGAAATCG	28.6	34.6	52.3	57.4	430-463 bp	1.0	47.0
rps15-ycf1_p2	TTCTTGAAATGAAATGAATGCA	TGGTTTAATACTACTAACGGAAATCG	27.3	34.6	53.2	57.4	431-464 bp	1.0	47.0
rps15-ycf1_p3	TTTCTTGAAATGAAATGAATGCA	TGGTTTAATACTACTAACGGAAATCG	26.1	34.6	54.0	57.4	432-465 bp	1.0	47.0
rps15-ycf1_p4	TCTTGAAATGAAATGAATGCAT	TGGTTTAATACTACTAACGGAAATCG	27.3	34.6	53.0	57.4	430-463 bp	1.0	47.0
rps15-ycf1_p5	TCTTGAAATGAAATGAATGCA	TTGGTTTAATACTACTAACGGAAATCG	28.6	33.3	52.3	57.9	431-464 bp	1.0	47.0